Evidence map›Paper›PMID 41331326›Full record

ArticleCommunications biology2025

Multi-modal label-free imaging of cellular metabolism and oxidative stress in 3D brain tissue models.

Yang Zhang, Maria Savvidou, Volha Liaudanskaya, Varshini Ramanathan, Thi Bui, Matthew Lindley, Yuhang Fu, Ash Sze, Ugochukwu O Ugwu, Matthew E Dilsizian and 6 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Communications biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Yang ZhangDepartment of Biomedical Engineering, Tufts University, Medford, MA, USA.ORCID http://orcid.org/0000-0003-0549-9202
Maria SavvidouDepartment of Biomedical Engineering, Tufts University, Medford, MA, USA.
Volha LiaudanskayaDepartment of Biomedical Engineering, Tufts University, Medford, MA, USA.ORCID http://orcid.org/0000-0002-9409-0136
Varshini RamanathanDepartment of Biomedical Engineering, Tufts University, Medford, MA, USA.
Thi BuiDepartment of Biomedical Engineering, Tufts University, Medford, MA, USA.
Matthew LindleyDepartment of Biomedical Engineering, Tufts University, Medford, MA, USA.ORCID http://orcid.org/0000-0002-8164-8479
Yuhang FuDepartment of Biomedical Engineering, Tufts University, Medford, MA, USA.
Ash SzeDepartment of Biomedical Engineering, Tufts University, Medford, MA, USA.
Ugochukwu O UgwuDepartment of Biomedical Engineering, Tufts University, Medford, MA, USA.
Matthew E DilsizianDepartment of Biomedical Engineering, Tufts University, Medford, MA, USA.
Xinjie ChenDepartment of Biomedical Engineering, Tufts University, Medford, MA, USA.
Sevara NasritdinovaDepartment of Biomedical Engineering, Tufts University, Medford, MA, USA.
Aonkon DeyDepartment of Biomedical Engineering, Tufts University, Medford, MA, USA.
Eric L MillerDepartment of Electrical and Computer Engineering, Tufts University, Medford, MA, USA.
David L KaplanDepartment of Biomedical Engineering, Tufts University, Medford, MA, USA.ORCID http://orcid.org/0000-0002-9245-7774
Irene GeorgakoudiDepartment of Biomedical Engineering, Tufts University, Medford, MA, USA. irene.georgakoudi@dartmouth.edu.ORCID http://orcid.org/0000-0002-0049-6697

Funding

Translational Engineering in Cancer (TEC)P30CA023108 · NCI · DARTMOUTH COLLEGE · PI Fred W Kolling IV · 1985 to 2026
$91.3M
Tissue Engineering Resource Center: TTDP41EB027062 · NIBIB · COLUMBIA UNIVERSITY HEALTH SCIENCES · PI Gordana Vunjak-Novakovic · 2019 to 2026
$12.6M
Probe-based two photon microscopy for functional, label-free early cancer diagnosisR01EB030061 · NIBIB · TUFTS UNIVERSITY MEDFORD · PI BEN-YAKAR, ADELA, GEORGAKOUDI, IRENE · 2020 to 2023
$3.2M
Acquisition of Leica confocal/multiphoton microscopeS10OD021624 · OD · TUFTS UNIVERSITY MEDFORD · PI GEORGAKOUDI, IRENE · 2016 to 2016
$600k
NCI NIH HHS P30 CA023108NIBIB NIH HHS P41 EB027062NIBIB NIH HHS R01 EB030061NIH HHS S10 OD021624U.S. Department of Defense (United States Department of Defense) HU0001-20-2-0015U.S. Department of Defense (United States Department of Defense) W81XWH2211065U.S. Department of Defense (United States Department of Defense) W911NF-23-1-0276U.S. Department of Health & Human Services | National Institutes of Health (NIH) P30CA023108U.S. Department of Health & Human Services | National Institutes of Health (NIH) P41EB027062U.S. Department of Health & Human Services | National Institutes of Health (NIH) R01EB030061U.S. Department of Health & Human Services | National Institutes of Health (NIH) S10OD021624
6 · The paper itself

Abstract

Label-free, two-photon fluorescence imaging enables non-destructive, single-cell metabolic assessments that capture metabolic reprogramming and heterogeneity. Here, we use intensity and lifetime imaging to report on cell metabolic function and dynamic interactions in 3D engineered brain tissue models, comprising human induced neural stem cells, astrocytes and microglia. We rely on spectral imaging to define excitation/emission profiles of key fluorophores, including NAD(P)H, lipoamide dehydrogenase, FAD, and lipofuscin. These profiles inform a multi-wavelength imaging strategy that accounts for lipofuscin and spectral overlap, ensuring accurate redox state, mitochondrial fragmentation, and NAD(P)H bound state analysis. Intensity imaging over 11 weeks reveals metabolic differences between neurons and astrocytes, highlighting glial support for neuronal function and oxidative stress mitigation. Lifetime measurements provide insights into NADPH utilization and antioxidant pathways. Collectively, our results support multi-modal, label-free optical imaging for assessing dynamic brain cell metabolism and interactions, laying the foundation for studying metabolic and mitochondrial dysfunction in brain disorders.

Indexed as

BrainOxidative StressAstrocytesHumansMicrogliaMicroscopy, Fluorescence, MultiphotonMitochondriaNADPNeural Stem CellsNeuronsOptical ImagingNADP

Identifiers

PMID41331326
PMCPMC12673076

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.