Evidence map›Paper›PMID 41332718›Full record

ArticlebioRxiv : the preprint server for biology2025

Structure and mechanism of microtubule stabilization and motor regulation by MAP9.

Burak Cetin, Aryan Taheri, Mert Golcuk, Brigette Y Monroy, Jonathan Fernandes, Kassandra M Ori-McKenney, Mert Gur, Eva Nogales, Ahmet Yildiz

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Burak CetinDepartment of Molecular and Cell Biology, University of California, Berkeley, CA, USA.
Aryan TaheriDepartment of Molecular and Cell Biology, University of California, Berkeley, CA, USA.
Mert GolcukDepartment of Computational and Systems Biology, University of Pittsburgh, Pittsburgh, PA, USA.
Brigette Y MonroyDepartment of Molecular and Cellular Biology, University of California, Davis, CA, USA.
Jonathan FernandesDepartment of Chemistry, University of California, Berkeley, CA, USA.
Kassandra M Ori-McKenneyDepartment of Molecular and Cellular Biology, University of California, Davis, CA, USA.
Mert GurDepartment of Computational and Systems Biology, University of Pittsburgh, Pittsburgh, PA, USA.
Eva NogalesDepartment of Molecular and Cell Biology, University of California, Berkeley, CA, USA.
Ahmet YildizDepartment of Molecular and Cell Biology, University of California, Berkeley, CA, USA.

Funding

Structural studies of function and regulation of microtubules and transcriptional gene expression machineryR35GM127018 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI Eva Nogales · 2018 to 2026
$4.3M
Defining microtubule cytoskeleton regulatory pathways in development and diseaseR35GM133688 · NIGMS · UNIVERSITY OF CALIFORNIA AT DAVIS · PI Kassandra Marie Ori-McKenney · 2019 to 2026
$3.1M
Understanding the Mechanism and Regulation of the Human Cytoplasmic Dynein ComplexR01GM094522 · NIGMS · UNIVERSITY OF CALIFORNIA BERKELEY · PI YILDIZ, AHMET · 2011 to 2019
$2.8M
NIGMS NIH HHS R01 GM094522NIGMS NIH HHS R35 GM127018NIGMS NIH HHS R35 GM133688
6 · The paper itself

Abstract

Microtubule-associated proteins (MAPs) regulate the organization of microtubules and control intracellular transport, but their individual contributions to microtubule dynamics and motor regulation remain poorly understood. Here, we identify MAP9 as a critical factor that stabilizes microtubules and facilitates neuronal morphogenesis. MAP9 knockdown abolishes the outgrowth of neurites, a phenotype not observed through the loss of other neuronal MAPs. Cryo-electron microscopy revealed that, unlike other MAPs that bind along protofilaments, MAP9 binds around the microtubule as a long alpha helix using five consecutive repeats. This unique binding mode enables MAP9 to staple adjacent protofilaments, thereby preventing microtubule depolymerization. We also showed that MAP9 selectively permits kinesin-3 motility while hindering kinesin-1 through interactions with a divergent loop-8 of their motor domains. Our results establish MAP9 as a key MAP required for neuronal growth and uncover how it differentially regulates intracellular transport driven by kinesin motors.

Identifiers

PMID41332718
PMCPMC12667982

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.