Evidence map›Paper›PMID 41335164›Full record

ArticleInflammopharmacology2026

Citral impairs intestinal changes caused by ulcerative colitis through modulation of antioxidant, anti-inflammatory and healing activities.

Maycon T Emílio-Silva, Vinícius P Rodrigues, Antonio J Ruiz-Malagon, Isabela G Guidolin, Felipe L Dario, Mariana M Fioravanti, Alba Rodriguez-Nogales, Julio Gálvez, Clelia A Hiruma-Lima

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Article in Inflammopharmacology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Maycon T Emílio-SilvaDepartment of Structural and Functional Biology, Physiology Sector, Institute of Bioscience, São Paulo State University, Botucatu, São Paulo, Brazil. maycon.silva@unesp.br.ORCID http://orcid.org/0000-0001-5466-3414
Vinícius P RodriguesDepartment of Structural and Functional Biology, Physiology Sector, Institute of Bioscience, São Paulo State University, Botucatu, São Paulo, Brazil.
Antonio J Ruiz-MalagonDepartment of Pharmacology, Center for Biomedical Research (CIBM), University of Granada, Granada, Spain.
Isabela G GuidolinDepartment of Structural and Functional Biology, Physiology Sector, Institute of Bioscience, São Paulo State University, Botucatu, São Paulo, Brazil.
Felipe L DarioDepartment of Structural and Functional Biology, Physiology Sector, Institute of Bioscience, São Paulo State University, Botucatu, São Paulo, Brazil.
Mariana M FioravantiDepartment of Structural and Functional Biology, Physiology Sector, Institute of Bioscience, São Paulo State University, Botucatu, São Paulo, Brazil.
Alba Rodriguez-NogalesDepartment of Pharmacology, Center for Biomedical Research (CIBM), University of Granada, Granada, Spain.
Julio GálvezDepartment of Pharmacology, Center for Biomedical Research (CIBM), University of Granada, Granada, Spain.
Clelia A Hiruma-LimaDepartment of Structural and Functional Biology, Physiology Sector, Institute of Bioscience, São Paulo State University, Botucatu, São Paulo, Brazil.

Funding

Conselho Nacional de Desenvolvimento Científico e Tecnológico 31157/2020-0FAPESP 2020/15225-1FAPESP 2021/11110-8FAPESP 2023/13172-6FAPESP 2024/08754-9FAPESP 2024/15790-1
6 · The paper itself

Abstract

introductionUlcerative colitis (UC) is the main representative of inflammatory bowel diseases (IBD) are chronic conditions characterized by intestinal inflammation, caused by the overproduction of pro-oxidant species and an immune response that damages the gut mucosa.

objectiveTo evaluate the anti-inflammatory and protective effect of Citral, a monoterpene, on in vitro and in vivo models of UC. METHODOLOGY: Male C57BL/6J mice were used for DSS 3%-induced UC for 5 days in drinking water. Concomitantly, daily oral citral (25, 100, and 300 mg/kg, p.o.) or vehicle was administered. Colonic segments were collected to evaluate neutrophil infiltration, lipid peroxidation, and the expression of antioxidant markers. NCM-356 and RAW-294 cells were stimulated with LPS (10 and 100 ng/mL, respectively). Cell viability, nitrite levels, scratch wound healing assay, and gene expression of inflammatory and growth factors were assessed.

resultsAcute treatment with citral (100 and 300 mg/kg) exhibited an anti-colitis effect by reducing the disease activity index (DAI) score compared to that in the DSS-vehicle group. This response was mediated by a significant reduction in myeloperoxidase activity and thiobarbituric acid reactive species levels, associated with an increase in superoxide dismutase activity, indicating anti-inflammatory and antioxidant activities (p < 0.05). The monoterpene reversed LPS-induced intestinal epithelial disturbance in the scratch wound healing process under different conditions and possibly reduced inos expression.

conclusionCitral treatment prevents the development of UC via anti-inflammatory, antioxidant, and healing effects.

Indexed as

Acyclic MonoterpenesAnti-Inflammatory AgentsAntioxidantsColitis, UlcerativeMonoterpenesAnimalsCell SurvivalDextran SulfateDisease Models, AnimalIntestinal MucosaLipid PeroxidationLipopolysaccharidesMaleMiceMice, Inbred C57BLWound HealingAcyclic MonoterpenesAnti-Inflammatory AgentsAntioxidantscitralDextran SulfateLipopolysaccharidesMonoterpenesAnti-inflammatoryInflammatory bowel diseasesLPSMonoterpeneOxidative stress

Identifiers

PMID41335164

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.