Evidence mapPaperPMID 41339487Full record

ArticleScientific reports2025

Automated cardiac MRI analysis for robust profiling of heart failure models in mice.

Thulaciga Yoganathan, Matt Sooknah, Baby Martin-McNulty, James Lee, Florian Schmid, Austin Lefebvre, Frank Kober, Johannes Riegler

Abstract read
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Thulaciga YoganathanCalico Life Sciences LLC, 1170 Veterans Blvd, South San Francisco, CA, 94080, USA. thulacigayoganathan@calicolabs.com.
Matt SooknahCalico Life Sciences LLC, 1170 Veterans Blvd, South San Francisco, CA, 94080, USA.
Baby Martin-McNultyCalico Life Sciences LLC, 1170 Veterans Blvd, South San Francisco, CA, 94080, USA.
James LeeCalico Life Sciences LLC, 1170 Veterans Blvd, South San Francisco, CA, 94080, USA.
Florian SchmidCalico Life Sciences LLC, 1170 Veterans Blvd, South San Francisco, CA, 94080, USA.
Austin LefebvreCalico Life Sciences LLC, 1170 Veterans Blvd, South San Francisco, CA, 94080, USA.
Frank KoberAix-Marseille Univ, CNRS, Centre de Résonance Magnétique Biologique et Médicale (CRMBM), Marseille, France.
Johannes RieglerCalico Life Sciences LLC, 1170 Veterans Blvd, South San Francisco, CA, 94080, USA. riegler@calicolabs.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Heart failure with preserved ejection fraction (HFpEF) is a complex, age-related cardiovascular disease with limited treatment options, partly due to a poor understanding of underlying mechanisms, lack of robust preclinical models and diagnostic tools with limited specificity. Traditional cardiac magnetic resonance imaging (MRI) protocols and analysis in preclinical research are time-consuming, and manual analysis methods are prone to high inter-observer variability (correlation coefficient of 0.79 for left ventricular (LV) ejection fraction between observers). To accelerate and standardize phenotyping, we optimized a comprehensive non-contrast cardiac MRI protocol for high throughput, enabling acquisition of a stack of 12 short-axis slices in approximately seven minutes. This time-efficiency allowed us to add additional sequences, including cine-Arterial Spin Labeling (ASL) for myocardial perfusion mapping and dobutamine stress testing, allowing for a comprehensive cardiac exam. We developed a deep learning approach utilizing 3D Convolutional Neural Networks (CNNs) for fully automated segmentation and quantification of cardiac function. We validated this comprehensive pipeline in two multifactorial mouse models of HFpEF, combining diet-induced obesity (DIO) or high fat diet (HFD) and the hypertensive agent, deoxycorticosterone pivalate (DOCP). Our approach illustrated high technical sensitivity by detecting significant myocardial perfusion reduction in both the DIO (p = 0.02) and DIO + DOCP (p = 0.03) groups compared to control, along with subtle diastolic abnormalities, even in the absence of overt changes in ejection fraction. The CNN demonstrated high accuracy and reproducibility, achieving a mean Dice similarity coefficient greater than 0.9 for segmentation and Intraclass Correlation Coefficients (ICC) exceeding 0.95 for key left ventricular functional parameters (volumes and mass) compared to expert consensus reads. This optimized protocol and automated analysis pipeline provides a valuable tool for preclinical cardiovascular research, enabling efficient and reliable assessment of cardiac remodeling and contributing to a deeper understanding of HFpEF progression.

Indexed as

HeartHeart FailureMagnetic Resonance ImagingAnimalsDiet, High-FatDisease Models, AnimalMagnetic Resonance Imaging, CineMaleMiceMice, Inbred C57BLStroke Volume

Identifiers

PMID41339487
PMCPMC12789537

What Socratic holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.