Evidence map›Paper›PMID 41345679›Full record

ArticleCell communication and signaling : CCS2025

Hypo-glycosylated FSH enhances the ovarian microenvironment for follicular development compared to fully glycosylated FSH.

Jiabin Tang, Zihui Wu, Miaoyu Liu, Linghua Xu, Jiarui Cheng, Chuanlong Wang, Xuedan Zhu, Xinrui Zhou, Liguo Yang, John S Davis and 1 more

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell communication and signaling : CCS, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Jiabin TangCollege of Animal Science and Technology, Key Laboratory of Agriculture Animal Genetics, Breeding and Reproduction, Huazhong Agriculture University, Ministry of Education, Wuhan, 430070, Hubei, P.R. China.
Zihui WuCollege of Animal Science and Technology, Key Laboratory of Agriculture Animal Genetics, Breeding and Reproduction, Huazhong Agriculture University, Ministry of Education, Wuhan, 430070, Hubei, P.R. China.
Miaoyu LiuCollege of Animal Science and Technology, Key Laboratory of Agriculture Animal Genetics, Breeding and Reproduction, Huazhong Agriculture University, Ministry of Education, Wuhan, 430070, Hubei, P.R. China.
Linghua XuCollege of Animal Science and Technology, Key Laboratory of Agriculture Animal Genetics, Breeding and Reproduction, Huazhong Agriculture University, Ministry of Education, Wuhan, 430070, Hubei, P.R. China.
Jiarui ChengCollege of Animal Science and Technology, Key Laboratory of Agriculture Animal Genetics, Breeding and Reproduction, Huazhong Agriculture University, Ministry of Education, Wuhan, 430070, Hubei, P.R. China.
Chuanlong WangCollege of Animal Science and Technology, Key Laboratory of Agriculture Animal Genetics, Breeding and Reproduction, Huazhong Agriculture University, Ministry of Education, Wuhan, 430070, Hubei, P.R. China.
Xuedan ZhuCollege of Animal Science and Technology, Key Laboratory of Agriculture Animal Genetics, Breeding and Reproduction, Huazhong Agriculture University, Ministry of Education, Wuhan, 430070, Hubei, P.R. China.
Xinrui ZhouCollege of Animal Science and Technology, Key Laboratory of Agriculture Animal Genetics, Breeding and Reproduction, Huazhong Agriculture University, Ministry of Education, Wuhan, 430070, Hubei, P.R. China.
Liguo YangCollege of Animal Science and Technology, Key Laboratory of Agriculture Animal Genetics, Breeding and Reproduction, Huazhong Agriculture University, Ministry of Education, Wuhan, 430070, Hubei, P.R. China.
John S DavisOlson Center for Women's Health, Department of Obstetrics and Gynecology, University of Nebraska Medical Center, Omaha, NE, 68198, USA.
Guohua HuaCollege of Animal Science and Technology, Key Laboratory of Agriculture Animal Genetics, Breeding and Reproduction, Huazhong Agriculture University, Ministry of Education, Wuhan, 430070, Hubei, P.R. China. huaguohua@mail.hzau.edu.cn.

Funding

The Aging Pituitary/Gonadal AxisP01AG029531 · NIA · WICHITA STATE UNIVERSITY · PI GEORGE R BOUSFIELD · 2009 to 2026
$25.9M
Understanding The Role Of FSHR Oligomerization And Trafficking In Transducing Age-dependent Changes In FSH GlycoformsR01HD113570 · NICHD · KING'S COLLEGE LONDON · PI JOHN S DAVIS, KIM CAROL JONAS · 2024 to 2026
$1.1M
BLRD VA IK6 BX005797National Institutes of Health, United States AG029531National Key R&D Program of China 2023YFD1300604National Natural Science Foundation of China 32172732National Natural Science Foundation of China 32472907NIA NIH HHS P01 AG029531NICHD NIH HHS R01 HD113570VA Senior Research Career Scientist Award, United States BX005797
6 · The paper itself

Abstract

Differential N-glycosylations of follicle-stimulating hormone (FSH) influence follicular development dynamics, significantly affecting assisted reproductive technology outcomes. However, the ovarian microenvironment induced by different FSH glycoforms remains incompletely understood. In this study, in vivo analyses using RT-qPCR, single-cell RNA sequencing, and bulk RNA sequencing demonstrated that FSH administration rapidly activated responses in both granulosa cells (GCs) and theca cells (TCs) within 2 h. Hypo-glycosylated FSH (hgFSH) initiated GCs and TCs differentiation more effectively than fully glycosylated FSH (fgFSH), and genes with higher expression in hgFSH were predominantly located in these cell types. HgFSH significantly upregulated 20.2% of total ovarian ligand genes, suggesting a more actively ovarian communicating microenvironment. In contrast, fgFSH inhibited the expression of oocyte-specific ligands such as Bmp15, Gdf9 and Oosp1, potentially disrupting oocytes-GCs communication. More importantly, hypo-glycosylated FSH upregulated genes significantly enriched in TCs. HgFSH activated key biological pathways in TCs such as lipid metabolic process and enhanced communication between GCs and TCs through more interactions than fgFSH. Our findings suggest that hgFSH fosters a more effective ovarian microenvironment for follicular development compared to fgFSH, highlighting the potential clinical benefits of hgFSH applications.

Indexed as

Cellular MicroenvironmentFollicle Stimulating HormoneOvarian FollicleOvaryAnimalsFemaleGlycosylationGranulosa CellsMiceOocytesTheca CellsFollicle Stimulating HormoneCell-cell communicationDifferent glycoformsFSHGranulosa cellsOocytesTheca cells

Identifiers

PMID41345679
PMCPMC12797953

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.