ArticleProteomics2026
High-Resolution Ion Mobility as an Alternative to Quadrupole-Based Precursor Isolation for Reducing Chimeric Fragmentation Spectra in Bottom-Up Proteomics.
Article in Proteomics, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed.
- A Parallel Accumulation-Mobility Aligned Fragmentation Strategy Utilizing High-Resolution Ion Mobility for High-Performance Proteomics Analysis.Molecular & cellular proteomics : MCP · 2026Article
- xTracer: Integrating chromatogram and mobiligram correlations for untargeted peptide identification in SLIM-based PAMAF data.bioRxiv : the preprint server for biology · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To assess the potential for high resolution ion mobility (HRIM) as an alternative means of precursor isolation for mass spectrometry fragmentation analysis we performed a meta-analysis of predicted tryptic peptide features from the human proteome to measure the rate of chimeric spectrum generation relative to traditional quadrupole-based isolation. Results indicate that for proteomic mixtures, HRIM separation with a peak capacity of 100 produces chimeric spectra at a rate commensurate with a ∼5 Th quadrupole isolation window, while providing the additional benefit of generating non-chimeric spectra for many isobaric and isomeric peptides unresolvable with a quadrupole filter. This behavior was verified experimentally using a HRIM-QTOF mass spectrometry system. The ability to combine HRIM and MS isolation resulted in >10× increase in precursor isolation specificity as compared to either of the techniques independently.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.