Uricase-free and cost-effective colorimetric assay for uric acid based on CoBTC metal-organic frameworks.

Shokooh Sadat Khaloo, Mohammad Hossein Vaziri, Fatemeh Azizi

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Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

3 authors.

Shokooh Sadat KhalooWorkplace Health Promotion Research Center, Research Institute for Health Sciences and Environment, Shahid Beheshti University of Medical Sciences, Tehran, Iran. sh_khaloo@sbmu.ac.ir.

Mohammad Hossein VaziriWorkplace Health Promotion Research Center, Research Institute for Health Sciences and Environment, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Fatemeh AziziDepartment of Occupational Hygiene Engineering, School of Public Health and Safety, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Funding

Shahid Beheshti University of Medical Sciences 43006286

6 · The paper itself

Abstract

The development of enzyme-free uric acid (UA) detection methods remains challenging due to cost, stability, and sensitivity limitations. Here, we report a uricase-free colorimetric biosensor using cobalt-1,3,5-benzene tricarboxylate metal-organic frameworks (CoBTC MOFs) with intrinsic peroxidase-like activity. CoBTC catalyzes the oxidation of TMB by conducting H₂O₂ to blue oxTMB, while UA reduces oxTMB to colorless TMB, allowing for the quantification of UA via absorbance changes. This biosensor achieved a detection limit of 0.25 μM and a linear range of 0.4-180 μM, outperforming many uricase-dependent assays. Clinical validation with human serum (recovery: 98-103%, RSD < 3%) confirmed the resistance to matrix effects. This work provides a scalable and low-cost alternative for point-of-care UA monitoring in diagnostic settings.

Indexed as

Biosensing TechniquesCobaltColorimetryMetal-Organic FrameworksUric AcidCost-Benefit AnalysisHumansHydrogen PeroxideLimit of DetectionOxidation-ReductionUrate OxidaseCobaltHydrogen PeroxideMetal-Organic FrameworksUrate OxidaseUric AcidClinical diagnosticsColorimetricEnzyme-freeMOF-based nanozymeUric acid detection

Identifiers

PMID41350306

DOI10.1038/s41598-025-27260-x

PMCPMC12680648

What Socratic holds

Textmetadata

LicenceCC BY-NC-ND

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.