Evidence mapPaperPMID 41350499Full record

ReviewOdontology2026

Platelet-rich fibrin-derived extracellular vesicles: emerging biological mediators in periodontal regeneration.

Halil Ata Bıçakçıoğlu, Gülenay Çolak

Abstract readReview
PubMed Publisher
In one paragraph

Review in Odontology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Halil Ata BıçakçıoğluDepartment of Periodontology, Faculty of Dentistry, Gazi University, Emek, 06490, Ankara, Turkey. ata.bicakcioglu@icloud.com.ORCID http://orcid.org/0000-0002-2962-0022
Gülenay ÇolakDepartment of Periodontology, Faculty of Dentistry, Gazi University, Emek, 06490, Ankara, Turkey.ORCID http://orcid.org/0000-0002-1171-2228

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Platelet-rich fibrin (PRF) is an autologous biomaterial increasingly used in periodontal regeneration due to its ability to release bioactive molecules in a sustained manner. Recent research indicates that extracellular vesicles (EVs) released during PRF clot formation may serve as pivotal paracrine mediators, orchestrating immune modulation and tissue healing. This review provides a comprehensive overview of the biogenesis, molecular cargo, immunomodulatory activity, and regenerative potential of PRF-derived extracellular vesicles (PRF-EVs), with emphasis on their translational relevance in periodontal therapy. A narrative analysis was conducted using PubMed, Scopus, and Web of Science databases (2018-2025). Studies investigating EV isolation, characterization, immunoregulatory mechanisms, and regenerative applications related to PRF or platelet-derived EVs were critically evaluated in accordance with the MISEV2023 guidelines. PRF-EVs encapsulate growth factors (TGF-β1, VEGF, PDGF-A), matrix-regulating proteins (MMP-9, thrombospondin-1), and regulatory microRNAs (miR-21, miR-146a). These vesicles promote macrophage polarization toward the M2 phenotype, suppress NF-κB activation, rebalance the Th17/Treg axis, and enhance fibroblast migration, angiogenesis, and osteogenesis. Comparative analyses reveal that while mesenchymal stem cell-derived EVs exhibit higher bioengineering potential, PRF-EVs exhibit promising biosafety and clinical feasibility; however, existing human trials remain limited in scale and duration, and long-term safety data are still required. Integration with hydrogels or 3D-printed scaffolds further enables controlled release and targeted local action. PRF-EVs constitute a cell-free, autologous, and biologically active platform that bridges immune regulation and regenerative healing. Future studies should focus on standardizing isolation and dosing protocols, applying AI-assisted quality control, and conducting multicenter clinical trials to ensure reproducibility and translational reliability in periodontal regeneration. These findings provide a biological and technological rationale supporting the potential application of PRF-EVs in evidence-based periodontal regenerative therapy.

Indexed as

Extracellular VesiclesGuided Tissue Regeneration, PeriodontalPlatelet-Rich FibrinRegenerationHumansExtracellular vesiclesImmunomodulationPeriodontal regenerationPlatelet-rich fibrinTissue engineeringTranslational dentistry

Identifiers

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.