Evidence map›Paper›PMID 41350613›Full record

ArticleBMC genomics2025

Generation and transcriptome profiling of bonobo induced pluripotent stem cells using stealth RNA vectors: a tripartite comparative study with humans and chimpanzees.

Yusuke Hamazaki, Hiroto Akuta, Hikaru Suzuki, Hideyuki Tanabe, Tsubasa Suzuki, Kouki Inoue, Kenji Ichiyanagi, Takuya Imamura, Masanori Imamura

Abstract readComparative Study
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC genomics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Yusuke Hamazaki *Molecular Biology Section, Center for the Evolutionary Origins of Human Behavior, Kyoto University, Inuyama, 484-8506, Japan.
Hiroto Akuta *Laboratory of Molecular and Cellular Physiology, Graduate School of Integrated Sciences for Life, Hiroshima University, Higashi-Hiroshima, 739-0046, Japan.
Hikaru SuzukiLaboratory of Genome and Epigenome Dynamics, Department of Animal Sciences, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, 464-8601, Japan.
Hideyuki TanabeResearch Center for Integrative Evolutionary Science, SOKENDAI (The Graduate University for Advanced Studies), Hayama, 240-0193, Japan.
Tsubasa SuzukiMolecular Biology Section, Center for the Evolutionary Origins of Human Behavior, Kyoto University, Inuyama, 484-8506, Japan.
Kouki InoueDepartment of Regulatory Biology, Faculty of Science, Saitama University, Saitama, 338-8570, Japan.
Kenji IchiyanagiLaboratory of Genome and Epigenome Dynamics, Department of Animal Sciences, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, 464-8601, Japan.
Takuya ImamuraLaboratory of Molecular and Cellular Physiology, Graduate School of Integrated Sciences for Life, Hiroshima University, Higashi-Hiroshima, 739-0046, Japan. timamura@hiroshima-u.ac.jp.
Masanori ImamuraMolecular Biology Section, Center for the Evolutionary Origins of Human Behavior, Kyoto University, Inuyama, 484-8506, Japan. imamura-masanori@staff.kanazawa-u.ac.jp.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Bonobos (Pan paniscus), which share a close evolutionary lineage with chimpanzees (Pan troglodytes) and humans, offer vital insights into the genetic and developmental propensities underlying human evolution. In this study, we established nine integration-free bonobo induced pluripotent stem cell (iPSC) lines from cryopreserved peripheral blood mononuclear cells using Sendai virus-derived RNA vectors. These iPSCs exhibited key pluripotency gene expression, normal karyotypes, and the capacity for differentiation into neural, mesodermal, and endodermal lineages, including the first successful induction of limb bud mesoderm cells from great ape iPSCs. Comprehensive transcriptome comparisons among bonobo, chimpanzee, and human iPSCs uncovered significant gene expression and retrotransposon divergence between Homo and Pan species, while highlighting minimal differences between bonobos and chimpanzees. Nevertheless, variation in the expression of mitochondrial metabolism-related genes was observed between the Pan species, suggesting potential interspecies differences in metabolic activity. This work provides a valuable platform for investigating the molecular and cellular mechanisms underlying species-specific developmental traits in closely related hominids and sheds light on the evolutionary origins of human characteristics.

Indexed as

Gene Expression ProfilingGenetic VectorsInduced Pluripotent Stem CellsPan paniscusPan troglodytesRNATranscriptomeAnimalsCell DifferentiationHumansRNABonoboChimpanzeeInduced pluripotent stem cellsMitochondrial metabolismRetrotransposonTranscriptome

Identifiers

PMID41350613
PMCPMC12797634

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.