ArticleBMC genomics2025
Integrated analyses reveal drought responsive microRNAs and their target genes in cucumber.
Article in BMC genomics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Abstract
Cucumber (Cucumis sativus), a key vegetable crop, is highly sensitive to water deficiency. MicroRNAs (miRNAs ) are small non-coding RNAs (18–25 nucleotides), which regulate gene expression via sequence-specific binding, and are crucial for plant growth, development, and stress responses. To explore drought-responsive miRNAs and their targets in C. sativus, transcriptome, miRNA, and degradome sequencing were conducted on drought-stressed cucumber leaves. A total of 7,871 differentially expressed mRNAs and 57 differentially expressed miRNAs (55 known and 2 novel) were identified. Degradome analysis revealed 43 miRNAs targeting 129 genes, forming 164 miRNA-target gene pairs, which were primarily involved in secondary metabolite biosynthesis and abiotic stress responses. Among them, 11 key miRNA-target gene pairs linked to drought response were highlighted. This study offered valuable molecular insights into miRNA-mediated drought regulation in cucumbers, thereby contributing our understanding of their stress adaptation mechanisms.
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