Evidence mapPaperPMID 41354813Full record

ArticleEuropean journal of medical research2025

LncRNA AC116914.2 promotes the progression of oral cancer by targeting METTL3 via miR-1245a/ZNF250.

Chunyu Feng, Xinyu Dou, Xiaoshuai Xu, Zihan Dang, Zihan Jiang, Erhui Jiang, Zhengjun Shang

Abstract read
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Article in European journal of medical research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Chunyu Feng *State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Hongshan District, 237 Luoyu Road, Wuhan, 430079, China.
Xinyu Dou *State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Hongshan District, 237 Luoyu Road, Wuhan, 430079, China.
Xiaoshuai XuDay Surgery Center, School and Hospital of Stomatology, Wuhan University, Wuhan, China.
Zihan DangState Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Hongshan District, 237 Luoyu Road, Wuhan, 430079, China.
Zihan JiangState Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Hongshan District, 237 Luoyu Road, Wuhan, 430079, China.
Erhui JiangState Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Hongshan District, 237 Luoyu Road, Wuhan, 430079, China. jiangerhui@whu.edu.cn.
Zhengjun ShangState Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan University, Hongshan District, 237 Luoyu Road, Wuhan, 430079, China. shangzhengjun@whu.edu.cn.

Funding

Key Research Project of Hubei Province 2023BCB135National Key R&D Program of China 2022YFC2504200National Natural Science Foundation of China 82103382
6 · The paper itself

Abstract

backgroundAberrant long non-coding RNA (lncRNA) expression regulates tumor progression. With rising incidence, oral squamous cell carcinoma (OSCC) requires mechanistic study. LncRNA functions in OSCC remain unclear.

methodsBioinformatics analysis was conducted to screen the highly expressed lncRNA AC116914.2 associated with m6A methylation in OSCC from The Cancer Genome Atlas database. Techniques including IHC, Western Blot, qPCR, cell functional assays, and in-vivo tumor formation were applied to assess the effects of AC116914.2 on OSCC malignant behaviors and fibroblast functions. Dual-luciferase assays explored the interactions among AC116914.2, miR-1245a, and ZNF250, while ChIP-qPCR verified ZNF250's regulatory effect on METTL3.

resultsLncRNA AC116914.2 showed differential expression between tumor and normal tissues. Its knockdown significantly inhibited OSCC progression in vitro and in vivo. Mechanistically, AC116914.2 competitively bound to miR-1245a to maintain ZNF250 levels, stabilizing the oncogene METTL3 expression. Additionally, AC116914.2 promoted extracellular vesicle release and activated cancer-associated fibroblasts, fostering an OSCC-favorable microenvironment.

conclusionThis study shows that lncRNA AC116914.2, as a competing endogenous RNA (ceRNA), regulates the expression of METTL3 by competitively binding to miR-1245a with ZNF250, thus promoting OSCC progression. The discovery of this regulatory axis provides new diagnostic markers and therapeutic targets for OSCC.

Indexed as

MethyltransferasesMicroRNAsMouth NeoplasmsRNA, Long NoncodingAnimalsCell Line, TumorCell ProliferationDisease ProgressionGene Expression Regulation, NeoplasticHumansMiceMice, NudeMethyltransferasesMETTL3 protein, humanMicroRNAsRNA, Long NoncodingCAFsCeRNAExtracellular vesiclesLncRNA AC116914.2METTL3OSCC

Identifiers

PMID41354813
PMCPMC12797727

What Socratic holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.