ArticleMolecular reproduction and development2025
Establishment and Characterization of Bovine Trophoblast Stem Cells Growing as Adherent Cells or Vesicles.
Article in Molecular reproduction and development, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
2 citing papers in PubMed.
- Livestock Blastoids: In Vitro Blastocyst Models for Embryogenesis and Reproductive Technologies.Reproduction in domestic animals = Zuchthygiene · 2026Review
- Establishment and Characterization of Bovine Trophoblast Stem Cells Growing as Adherent Cells or Vesicles.Molecular reproduction and development · 2025Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In ungulates, the development of the trophoblast lineage follows a peculiar process, as implantation is preceded by a relatively long period of trophoblast elongation, the molecular control of which is still not well known. Here, we have established novel bovine trophoblast stem cells (b2iTSCs) growing in a completely defined medium containing inhibitors of GSK3b and MEK, and WNT3 during establishment. These cells can grow either as adherent, or as vesicles in suspension. These fluid-filled vesicles show high proliferative capacity and apical polarity. Transcriptome analysis reveals that b2iTSCs are distinct from bovine embryonic fibroblasts and exhibit gene expression profiles related to lipid metabolism, steroid hormone synthesis, and nutrient absorption. The cells also express different Pregnancy Associated Glycoproteins, such as PAG11. Comparative transcriptome analysis shows that 2iTSCs have a signature intermediate between early trophectoderm and elongating trophoblast, expressing canonical trophoblast markers but not IFNt. Additionally, b2iTSCs can differentiate into binucleate cells in vitro and participate in the formation of bovine blastoïds when cocultured with bovine embryonic stem cells, demonstrating their potential for studying trophoblast development and function.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.