Evidence map›Paper›PMID 41359105›Full record

ReviewMolecular biomedicine2025

Single-cell sequencing and organoids: applications in organ development and disease.

Tiantian Li, Jianmei Yin, Yang Hao, Wei Gao, Qirong Li, Qiang Feng, Boqiang Tao, Ming Hao, Yanxin Liu, Chao Lin and 1 more

Abstract readReview
PubMed Full text at PMC Publisher
In one paragraph

Review in Molecular biomedicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Review
  2. Organoid technology in cancer research.Molecular biomedicine · 2026
    Review
  3. Article
  4. Article
  5. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Tiantian LiLaboratory Animal Center, College of Animal Science, Jilin University, Changchun, 130021, China.
Jianmei YinDepartment of Otorhinolaryngology, The First Hospital of Jilin University, Changchun, China.
Yang HaoLaboratory Animal Center, College of Animal Science, Jilin University, Changchun, 130021, China.
Wei GaoLaboratory Animal Center, College of Animal Science, Jilin University, Changchun, 130021, China.
Qirong LiLaboratory Animal Center, College of Animal Science, Jilin University, Changchun, 130021, China.
Qiang FengLaboratory Animal Center, College of Animal Science, Jilin University, Changchun, 130021, China.
Boqiang TaoLaboratory Animal Center, College of Animal Science, Jilin University, Changchun, 130021, China.
Ming HaoLaboratory Animal Center, College of Animal Science, Jilin University, Changchun, 130021, China.
Yanxin LiuLaboratory Animal Center, College of Animal Science, Jilin University, Changchun, 130021, China.
Chao LinSchool of Grain Science and Technology, Jilin Business and Technology College, Changchun, China.
Dongxu WangLaboratory Animal Center, College of Animal Science, Jilin University, Changchun, 130021, China. wang_dong_xu@jlu.edu.cn.ORCID 0000-0003-0464-1596

Funding

Jilin Province Development and Reform Commission Program ZKJCFGW2023015the Jilin University young teachers and students cross-disciplinary training project 2023-JCXK-08Wenzhou Science & Technology Bureau Basic Public Welfare Research Program Y20240006
6 · The paper itself

Abstract

The integration of single-cell sequencing and organoid technologies has been transformative for biomedical research, enabling investigations of organ development, disease mechanisms, and therapeutic innovation at even finer resolutions. Organoids serve as 3D in vitro models that replicate the structural and functional complexity of human tissues, while single-cell sequencing can resolve cellular heterogeneity, transcriptional dynamics, and lineage trajectories at high resolution. This review systematically explores the synergistic potential of these two technologies across multiple domains. First, it describes their application in studying the developmental mechanisms of organs including the brain, lungs, heart, liver, intestines, and kidneys, revealing key signaling pathways and cellular interaction networks. Then, it details their application in studying in vitro models of various diseases, including neurodegenerative disorders, genetic diseases, infectious diseases, metabolic syndrome, and tumors, advancing the in-depth analysis of pathological mechanisms. By leveraging patient-derived organoid biobanks, combining these two technologies can accelerate drug screening and precision, while utilizing transplantable tissue constructs to pioneer regenerative medicine strategies. This review also highlights the strengths of combining these two technologies in dynamically decoding cellular behavior and communication networks. By constructing physiologically relevant multifunctional research platforms, the integration of single-cell sequencing with organoid models will accelerate the elucidation of disease mechanisms and drive innovative breakthroughs in precision medicine and regenerative medicine. Looking ahead, the deep integration of single-cell sequencing with organoids, combined with cutting-edge technologies such as spatial transcriptomics and gene editing, will continue to propel life sciences toward a transformative leap from descriptive research to mechanism-driven, precision-oriented, and personalized approaches.

Indexed as

OrganogenesisOrganoidsSingle-Cell AnalysisAnimalsHumansPrecision MedicineRegenerative MedicineCancer researchDevelopmentDisease modelingOrganoidsSingle-cell sequencing

Identifiers

PMID41359105
PMCPMC12686334

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.