Evidence map›Paper›PMID 41360746›Full record

ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2026

Effectiveness of Multivitamins vs Folic Acid on Prevention of Neural Tube Defects in Mouse Genetic Models and Human Organoids.

Huili Li, Jing Zhang, Lori Bulwith, Lee Niswander

Abstract read
In one paragraph

Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Huili LiMolecular, Cellular, Developmental Biology Department, University of Colorado, Boulder, 80309, USA.ORCID https://orcid.org/0000-0002-9248-0720
Jing ZhangMolecular, Cellular, Developmental Biology Department, University of Colorado, Boulder, 80309, USA.ORCID https://orcid.org/0000-0001-5901-6216
Lori BulwithMolecular, Cellular, Developmental Biology Department, University of Colorado, Boulder, 80309, USA.
Lee NiswanderMolecular, Cellular, Developmental Biology Department, University of Colorado, Boulder, 80309, USA.ORCID https://orcid.org/0000-0002-9959-0594

Funding

Project II - Modeling meningomyelocele in frog using human alleles and folic acid exposureP01HD104436 · NICHD · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI NISWANDER, LEE A. · 2021 to 2025
$7.0M
NICHD NIH HHS P01 HD104436
6 · The paper itself

Abstract

Maternal consumption of folic acid (FA)-containing multivitamins/minerals (MVM) are recommended to reduce the risk for neural tube defects (NTDs). However, comparison between MVM supplementation and FA alone relative to NTD risk and possible mechanisms remain unclear. The studies demonstrate MVM can decrease NTD incidence in mouse genetic models with FA-resistant or FA-detrimental responses. To evaluate cellular and metabolic impacts, human iPSC-derived neuroectoderm organoids are generated with an elliptical shape resembling the human neural tube and cranial to upper spinal identity. Upon pharmacological or genetic disruption, both MVM or FA alone can normalize abnormal apical F-actin, lumen size, and premature neuronal differentiation. However, FA alone enhances DNA synthesis and shortens the cell division rate, while MVM maintains these parameters at control levels. High-performance liquid chromatography (HPLC) analysis of mouse embryos and human organoids indicates FA causes more variation of the nucleotide pool, whereas MVM maintains homeostasis. Thymine is reduced in FA alone and increased in MVM and thymine/thymidine supplementation can ameliorate FA-induced hyperactivation of cell division. The in vivo cellular and phenotypic data in human organoids and mice show the effectiveness of MVM supplementation, that in some cases surpasses FA alone, in maintaining cellular homeostasis and preventing NTD.

Indexed as

Folic AcidNeural Tube DefectsOrganoidsVitaminsAnimalsDietary SupplementsDisease Models, AnimalFemaleHumansInduced Pluripotent Stem CellsMiceFolic AcidVitaminsfolic acidhuman neural tube organoidsmitosismultivitaminneural tube defect

Identifiers

PMID41360746
PMCPMC12884776

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.