Evidence map›Paper›PMID 41364201›Full record

ArticleJournal of materials science. Materials in medicine2025

LR-PRP-saturated bioactive bovine-derived decellularized pericardium accelerates osteogenesis in a rabbit bone defect model.

Mahmoud M Abouelfetouh, Hao Li, Eman Salah, Qianghui Lei, Ruiling Yin, Jiao Yaya, Peng Wang, Mingxing Ding, Huang Fei, Yi Ding

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of materials science. Materials in medicine, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Mahmoud M AbouelfetouhCollege of Veterinary Medicine, Huazhong Agricultural University, No.1, Shizishan Street, Hongshan District, Wuhan, Hubei Province, 430070, China.
Hao LiCollege of Veterinary Medicine, Huazhong Agricultural University, No.1, Shizishan Street, Hongshan District, Wuhan, Hubei Province, 430070, China.
Eman SalahDept of Pharmacology, Faculty of Veterinary Medicine, Benha University, Moshtohor, 13736, Egypt.
Qianghui LeiCollege of Veterinary Medicine, Huazhong Agricultural University, No.1, Shizishan Street, Hongshan District, Wuhan, Hubei Province, 430070, China.
Ruiling YinCollege of Veterinary Medicine, Huazhong Agricultural University, No.1, Shizishan Street, Hongshan District, Wuhan, Hubei Province, 430070, China.
Jiao YayaCollege of Veterinary Medicine, Huazhong Agricultural University, No.1, Shizishan Street, Hongshan District, Wuhan, Hubei Province, 430070, China.
Peng WangCollege of Veterinary Medicine, Huazhong Agricultural University, No.1, Shizishan Street, Hongshan District, Wuhan, Hubei Province, 430070, China.
Mingxing DingCollege of Veterinary Medicine, Huazhong Agricultural University, No.1, Shizishan Street, Hongshan District, Wuhan, Hubei Province, 430070, China.
Huang FeiWuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan, 430074, China. dfeihuang@163.com.
Yi DingCollege of Veterinary Medicine, Huazhong Agricultural University, No.1, Shizishan Street, Hongshan District, Wuhan, Hubei Province, 430070, China. dingyi@mail.hzau.edu.cn.

Funding

National Key R&D Program of China 2023YFD1801104-6
6 · The paper itself

Abstract

Combining decellularized biological scaffolds with PRP can prevent the rapid inactivation of growth factors and achieve their controlled and sustained release during tissue regeneration. Therefore, the purpose of this study was to evaluate the combined effect of decellularized bovine pericardium (dBP) and leukocyte-rich platelet-rich plasma (LR-PRP) on the bone repair in a rabbit femoral defect model. Bovine pericardium was decellularized using the Trypsin-Triton X-SDS protocol and histologically assessed. Unicortical bone defects were surgically created in the femur of rabbits (n = 6) and randomly assigned to three treatment allocations: (1) untreated control, (2) LR-PRP, and (3) LR-PRP + dBP-treated defects. Bone defect healing was evaluated using quantitative computed tomography (CT) and histopathological analyses. The dBP achieved 99.2% nucleus removal, retained about 34.3% of the sulfated glycosaminoglycan, and maintained a collagen content similar to the native pericardia. The CT voxel values of the defects treated with the LR-PRP + dBP increased by +33.2% and +56.2% at 2 and 4 weeks after surgery, respectively, compared to the control defects. As well, a significant rise in the CT values was observed in the LR-PRP + dBP treatment compared to the LR-PRP treatment alone at 2 weeks (p = 0.03) and 4 weeks (p = 0.02). Histopathologically, the LR-PRP + dBP treatment achieved higher bone repair scores with a significantly higher nascent bone area fraction (87.8%) compared to the LR-PRP (54%). These findings highlight the synergistic effect of dBP and LR-PRP, offering promising prospects in developing biocompatible scaffolds for enhancing bone repair.

Indexed as

OsteogenesisPericardiumPlatelet-Rich PlasmaAnimalsBone RegenerationCattleDisease Models, AnimalFemurRabbitsTissue EngineeringTissue ScaffoldsTomography, X-Ray ComputedComputed tomographyDecellularized bovine pericardiumHistopathological analysisLeukocyte-rich platelet rich plasmaOsteogenesis

Identifiers

PMID41364201
PMCPMC12714762

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.