Evidence mapPaperPMID 41366672Full record

ArticleBMC complementary medicine and therapies2025

Sweet orange essential oil and (+)-limonene prevent oxidative stress, reduce inflammation, and apoptosis in differentiated SH-SY5Y neuroblastoma/BV-2 microglia co-culture neurodegeneration models.

Edina Pandur, Loretta Heilmann, Margita Szilágyi-Utczás, Tibor Rák, Katalin Sipos, Adrienne Csutak, Györgyi Horváth

Abstract read
In one paragraph

Article in BMC complementary medicine and therapies, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

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4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Edina PandurDepartment of Pharmaceutical Biology, Faculty of Pharmacy, University of Pécs, Rókus Street 4, Pécs, Hungary.
Loretta HeilmannDepartment of Pharmaceutical Biology, Faculty of Pharmacy, University of Pécs, Rókus Street 4, Pécs, Hungary.
Margita Szilágyi-UtczásCenter for Sports Nutrition Science, Hungarian University of Sport Science, Alkotás Street 42-48, Budapest, Hungary.
Tibor RákDepartment of Pharmacognosy, Faculty of Pharmacy, University of Pécs, Rókus Street 4, Pécs, Hungary.
Katalin SiposDepartment of Pharmaceutical Biology, Faculty of Pharmacy, University of Pécs, Rókus Street 4, Pécs, Hungary.
Adrienne CsutakDepartment of Ophthalmology, University of Pécs, Medical School Clinical Centre, Rákóczi Street 2, Pécs, Hungary.
Györgyi HorváthDepartment of Pharmacognosy, Faculty of Pharmacy, University of Pécs, Rókus Street 4, Pécs, Hungary. horvath.gyorgyi@gytk.pte.hu.

Funding

University of Pécs, Faculty of Pharmacy, Clinical Center Collaboration Funding PTE GYTK-KK Kollab 2025
6 · The paper itself

Abstract

backgroundParkinson's disease (PD) is becoming increasingly prevalent worldwide. The pathophysiology of this condition is characterized by oxidative stress, inflammation, iron accumulation, mitochondrial dysfunction, and protein aggregation, all of which contribute to cell death and neurodegeneration. Microglia, the innate immune cells of the brain, play a significant role in the development and progression of PD by releasing inflammatory cytokines upon activation. Essential oils (EOs) are emerging as potential therapeutic agents because of their antioxidant and anti-inflammatory properties.

methodsIn our study, we investigated the antioxidant, anti-inflammatory, and anti-apoptotic effects of sweet orange [Citrus sinensis (L.) Osbeck (Rutaceae)] EO and its main compound (+)-limonene on monocultures of all-trans retinoic acid-differentiated and 6-hydroxydopamine-induced (6-OHDA) SH-SY5Y cells and bilaminar co-cultures containing differentiated and 6-hydroxydopamine-induced SH-SY5Y cells and BV-2 microglia.

resultsSweet orange EO and (+)-limonene significantly decreased reactive oxygen species (ROS) production and increased oxidative stress defense by increasing the total antioxidant capacity and glutathione peroxidase and superoxide dismutase activities in human neuroblastoma (SH-SY5Y) cells. Additionally, both EO and its main compound mitigated inflammation by downregulating the secretion of pro-inflammatory cytokines. They also decreased the cytochrome c levels and caspase-3 activity. Furthermore, sweet orange EO and (+)-limonene attenuated microglia-mediated inflammation in co-cultures, suggesting their potential application as modulators of microglial activity.

conclusionsBased on these results, sweet orange EO and its main component, (+)-limonene, can be considered as neuroprotective agents and are promising candidates for complementary therapy in Parkinson's disease.

Indexed as

Anti-Inflammatory AgentsApoptosisCitrus sinensisLimoneneMicrogliaOils, VolatileOxidative StressAnimalsAntioxidantsCell Line, TumorCoculture TechniquesHumansInflammationMiceNeuroblastomaNeuroprotective AgentsAnti-Inflammatory AgentsAntioxidantsLimoneneNeuroprotective AgentsOils, VolatileAntioxidant capacityCaspase-3(+)-limoneneOxidative stressPro-inflammatory cytokinesSweet orange essential oil

Identifiers

PMID41366672
PMCPMC12801818

What Socratic holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.