Evidence map›Paper›PMID 41367077›Full record

ArticleAnimal models and experimental medicine2026

Optimizing the dengue virus infection mouse model: Comparing different backgrounds and infection route for enhanced stability.

Dan Liao, Ming Zhong, Wenjiang Zheng, Zhendong Guo, Ye Zhou, Qiuhong Li, Lijuan Qiu, Liangwen Yu, Haishan Long, Geng Li

Abstract readComparative Study
In one paragraph

Article in Animal models and experimental medicine, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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2 · The registry

The trial behind it

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3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

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5 · Who and what money

Authors and funding

10 authors.

Dan LiaoChinese Medicine Guangdong Laboratory, Guangzhou University of Chinese Medicine, Zhuhai, China.ORCID 0009-0006-7256-1044
Ming ZhongExperimental Animal Center, Guangzhou University of Chinese Medicine, Guangzhou, China.
Wenjiang ZhengThe First Affiliated Hospital of Guangzhou University of Chinese Medicine, Guangzhou, China.
Zhendong GuoGuangdong Provincial Hospital of Traditional Chinese Medicine, Guangzhou, China.
Ye ZhouExperimental Animal Center, Guangzhou University of Chinese Medicine, Guangzhou, China.
Qiuhong LiExperimental Animal Center, Guangzhou University of Chinese Medicine, Guangzhou, China.
Lijuan QiuExperimental Animal Center, Guangzhou University of Chinese Medicine, Guangzhou, China.
Liangwen YuChinese Medicine Guangdong Laboratory, Guangzhou University of Chinese Medicine, Zhuhai, China.
Haishan LongExperimental Animal Center, Guangzhou University of Chinese Medicine, Guangzhou, China.
Geng LiChinese Medicine Guangdong Laboratory, Guangzhou University of Chinese Medicine, Zhuhai, China.

Funding

Guangdong Province Medical Research Fund Project B2024112Guangdong Province Natural Science Foundation - Guangzhou-South China Joint Youth Fund Project 2023A1515110849Special Project of the Research Platform of Guangdong Provincial Department of Traditional Chinese Medicine 20254040the Project of the Incubation Program for the Science and Technology Development of Chinese Medicine Guangdong Laboratory/ Hengqin Laboratory HQL2024PZ043The Scientific Research Special Project of the Joint Construction Project of High-level Hospitals between Guangzhou University of Chinese Medicine and the Scientific Research Fund Project GZYZS2024G09
6 · The paper itself

Abstract

backgroundIn recent decades, the global incidence of dengue fever has been steadily increasing, with continuous geographical expansion. Researchers have successfully modeled most clinical symptoms of human dengue fever using interferon type I (IFN-I) or combined IFN-I/II receptor knockout mice infected with dengue virus (DENV). However, this model requires further optimization to better support related studies.

methodsThis study aimed to establish a stable dengue infection model by evaluating the effects of different genetic backgrounds and injection routes on DENV infection in interferon receptor knockout mice. We first infected various strains of interferon receptor-deficient mice with DENV and compared their susceptibility based on clinical symptoms, viremia levels, organ indices, histopathological findings, and vascular leakage markers. Subsequently, we selected the most susceptible strain to further investigate the impact of different injection methods on infection outcomes.

resultsWe found that BALB/c background mice with type 1 interferon receptor knockout(IFNAR) had the most obvious symptoms. Subsequently, we selected IFNAR-/-BALB/c mice to further explore the effects of different injection methods on dengue virus infection. The results showed that the intraperitoneal injection group had the most severe clinical symptoms, the longest duration of viremia, and the most obvious degree of organ damage.

conclusionThrough systematic screening and optimization, we established a robust animal model of dengue virus infection via intraperitoneal injection in IFNAR-/- BALB/c mice. This model offers a valuable tool for future dengue research.

Indexed as

DengueDengue VirusDisease Models, AnimalAnimalsFemaleInjections, IntraperitonealMiceMice, Inbred BALB CMice, KnockoutReceptor, Interferon alpha-betaViremiaReceptor, Interferon alpha-betadengue feverIFNAR−/−BALB/c miceinterferon receptor knockoutmouse model

Identifiers

PMID41367077
PMCPMC12907974

What Socratic holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.