Evidence mapPaperPMID 41369340Full record

ArticleCells2025

Multimodal Imaging of the Corneal Endothelial Transition Zone Reveals Progenitor Cell Population.

Sonika Rathi, Patricia Hülse, Susanne Staehlke, Marcus Walckling, Mahmoud Anwar, Peter Trosan, Sebastian Bohn, Oliver Stachs, Gary S L Peh, Gary Hin-Fai Yam and 4 more

Abstract read
In one paragraph

Article in Cells, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Sonika RathiDepartment of Ophthalmology, Rostock University Medical Center, 18057 Rostock, Germany.
Patricia HülseDepartment of Ophthalmology, Rostock University Medical Center, 18057 Rostock, Germany.
Susanne StaehlkeDepartment of Ophthalmology, Rostock University Medical Center, 18057 Rostock, Germany.ORCID 0000-0002-0251-8263
Marcus WalcklingDepartment of Ophthalmology, Rostock University Medical Center, 18057 Rostock, Germany.
Mahmoud AnwarDepartment of Ophthalmology, Rostock University Medical Center, 18057 Rostock, Germany.ORCID 0000-0002-5884-6813
Peter TrosanDepartment of Ophthalmology, Rostock University Medical Center, 18057 Rostock, Germany.
Sebastian BohnDepartment of Ophthalmology, Rostock University Medical Center, 18057 Rostock, Germany.ORCID 0000-0001-9838-1769
Oliver StachsDepartment of Ophthalmology, Rostock University Medical Center, 18057 Rostock, Germany.
Gary S L PehRegenerative Therapy Group, Singapore Eye Research Institute, Singapore 169856, Singapore.ORCID 0000-0002-5387-4209
Gary Hin-Fai YamCorneal Regeneration Laboratory, University of Pittsburgh School of Medicine, Pittsburgh, PA 15219, USA.ORCID 0000-0002-8445-3669
Jodhbir S MehtaCorneal & External Eye Disease, Cataract & Comprehensive Ophthalmology, Refractive Surgery, Singapore National Eye Centre, Singapore 168751, Singapore.ORCID 0000-0001-6362-4898
Nicola HofmannGerman Society for Tissue Transplantation (DGFG), 30625 Hannover, Germany.ORCID 0000-0002-5281-1361
Martin BörgelGerman Society for Tissue Transplantation (DGFG), 30625 Hannover, Germany.ORCID 0009-0003-5900-943X
Thomas A FuchslugerDepartment of Ophthalmology, Rostock University Medical Center, 18057 Rostock, Germany.

Funding

DGFG (Deutsche Gesellschaft für Gewebetransplantation, Network for Tissue Donation, Tissue Processing, and Transplantation, Hannover and Rostock, Germany) P03_UMR_DGFG
6 · The paper itself

Abstract

The assessment of donor corneas is currently based solely on central endothelial cell (EC) density, which potentially overlooks the transition zone (TZ) regenerative potential. Therefore, the present study characterizes TZ using multimodal imaging techniques to understand its regenerative potential and refine the assessment of donor tissue. Ex vivo donor corneas (n = 41) were examined using phase-contrast microscopy for EC counting and reflectance confocal microscopy (HRTII/RCM) for non-invasive visualization of the TZ. A subset of eight of these corneas underwent ultrastructural analysis using field-emission scanning electron microscopy (SEM) and immunostaining analysis using confocal microscopy. We observed a significant decrease in central EC density (

Indexed as

CorneaEndothelium, CornealMultimodal ImagingStem CellsAdultAgedAged, 80 and overEndothelial CellsFemaleHumansMaleMiddle AgedTissue Donorsconfocal microscopycorneal endotheliumendothelial progenitor cellsstem cell markerstransition zone

Identifiers

PMID41369340
PMCPMC12691442

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.