Evidence map›Paper›PMID 41369405›Full record

ArticleCells2025

Extracellular Vesicles from iPSC-Derived Glial Progenitor Cells Prevent Glutamate-Induced Excitotoxicity by Stabilising Calcium Oscillations and Mitochondrial Depolarisation.

Margarita Shedenkova, Anastasiia Gurianova, Irina Krasilnikova, Anastasia Sudina, Evgeny Karpulevich, Yaroslav Maksimov, Marina Samburova, Egor Guguchkin, Zlata Nefedova, Valentina Babenko and 6 more

Abstract read
In one paragraph

Article in Cells, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Margarita ShedenkovaResearch Institute of Molecular and Cellular Medicine, Medical Institute of RUDN University, Moscow 117198, Russia.ORCID 0000-0001-7415-1520
Anastasiia GurianovaIvannikov Institute for System Programming of the Russian Academy of Sciences, Moscow 109004, Russia.
Irina KrasilnikovaNational Medical Research Center for Children's Health, Moscow 119991, Russia.ORCID 0000-0001-9453-3040
Anastasia SudinaResearch Institute of Molecular and Cellular Medicine, Medical Institute of RUDN University, Moscow 117198, Russia.ORCID 0000-0003-3531-7684
Evgeny KarpulevichIvannikov Institute for System Programming of the Russian Academy of Sciences, Moscow 109004, Russia.ORCID 0000-0002-6771-2163
Yaroslav MaksimovResearch Institute of Molecular and Cellular Medicine, Medical Institute of RUDN University, Moscow 117198, Russia.
Marina SamburovaResearch Institute of Molecular and Cellular Medicine, Medical Institute of RUDN University, Moscow 117198, Russia.ORCID 0009-0002-6816-1719
Egor GuguchkinIvannikov Institute for System Programming of the Russian Academy of Sciences, Moscow 109004, Russia.ORCID 0000-0001-7885-9892
Zlata NefedovaFSAEI HE I.M. Sechenov First MSMU of MOH of Russia, Sechenovskiy University, Moscow 119991, Russia.
Valentina BabenkoBelozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow 119991, Russia.
Daniil FrolovInstitute of Information Technology, Russian Technological University (RTU MIREA), Moscow 119454, Russia.ORCID 0000-0003-1081-1483
Kirill SavostyanovNational Medical Research Center for Children's Health, Moscow 119991, Russia.ORCID 0000-0003-4885-4171
Timur FatkhudinovResearch Institute of Molecular and Cellular Medicine, Medical Institute of RUDN University, Moscow 117198, Russia.ORCID 0000-0002-6498-5764
Dmitry GoldshteinResearch Institute of Molecular and Cellular Medicine, Medical Institute of RUDN University, Moscow 117198, Russia.
Zanda BakaevaNational Medical Research Center for Children's Health, Moscow 119991, Russia.ORCID 0000-0002-2797-3270
Diana SalikhovaResearch Institute of Molecular and Cellular Medicine, Medical Institute of RUDN University, Moscow 117198, Russia.ORCID 0000-0001-7842-7635

Funding

Russian Ministry of Education and Science KBK 075 0110 47 2 U8 70440 621
6 · The paper itself

Abstract

Neurodegenerative diseases pose a significant challenge to modern medicine. Despite significant advances in neurology, current therapeutic approaches often prove insufficient to treat such disorders. This study investigates the neuroprotective effect of extracellular vesicles derived from glial derivates of human-induced pluripotent stem cells. The extracellular vesicle's cargo was characterised by proteomic analysis. The neuroprotective effect was assessed using a model of glutamate excitotoxicity performed on a primary culture of cortical neuroglial cells. The viability of cells was estimated using the MTT test and morphometric analyses. A comprehensive methodology was applied to investigate intracellular mechanisms, integrating assessments of intracellular calcium concentrations, mitochondrial membrane potential, and targeted inhibition of the PI3K-Akt pathway. Transcriptomic analysis of neuroglial cultures was used to validate the role of obtained mechanisms of extracellular vesicle's neuroprotective effect. The obtaining results demonstrated the improvement of neuronal survival by reducing intracellular calcium levels and stabilising mitochondrial membrane potential under glutamate-induced excitotoxicity via PI3K-Akt signalling pathway activation. Moreover, the vesicles contained proteins that contribute to preventing apoptotic processes, activating regeneration of the nervous system, and modulating calcium ion transport and are associated with redox processes. Further transcriptomic analyses of neuroglial cultures treated with EVs showed an up-regulation of genes associated with regeneration, inhibition of calcium ion transport, regulation of membrane depolarisation, and negative regulation of apoptotic pathways.

Indexed as

Calcium SignalingExtracellular VesiclesGlutamic AcidInduced Pluripotent Stem CellsMembrane Potential, MitochondrialNeurogliaCalciumCell SurvivalHumansMitochondriaPhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktSignal TransductionCalciumGlutamic AcidPhosphatidylinositol 3-KinasesProto-Oncogene Proteins c-aktextracellular vesiclesglial progenitor cellsglutamateneuroprotectionproteometranscriptome

Identifiers

PMID41369405
PMCPMC12691032

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.