Evidence map›Paper›PMID 41370417›Full record

ArticleJBRA assisted reproduction2025

Impact of oxidative stress on female reproductive parameters: an analysis of systemic and follicular biomarkers.

Aline Q Rodrigues, Guilherme G Carvalho, Tathyana B Piau, Fabiane H Veiga, Paulo E N Souza, Daniel C Moreira, Natalia I Z Tierno, Yamara A Macedo, Maria Eduarda B Amaral, Hitomi M Nakagawa and 2 more

Abstract read
In one paragraph

Article in JBRA assisted reproduction, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Aline Q RodriguesUniversity of Brasilia, Institute of Biological Sciences, Department of Physiological Sciences, Brasilia-DF, 70910-900, Brazil.
Guilherme G CarvalhoUniversity of Brasilia, Institute of Biological Sciences, Department of Physiological Sciences, Brasilia-DF, 70910-900, Brazil.
Tathyana B PiauUniversity of Brasilia, Institute of Biological Sciences, Department of Physiological Sciences, Brasilia-DF, 70910-900, Brazil.
Fabiane H VeigaUniversity of Brasilia, Faculty of Health Sciences and Technologies, Ceilandia campus, Brasilia-DF, 70910-900, Brazil.
Paulo E N SouzaUniversity of Brasilia, Institute of Physics, Laboratory of Electron Paramagnetic Resonance, Brasilia-DF, 70910-900, Brazil.
Daniel C MoreiraUniversity of Brasilia, Faculty of Medicine, Morphology and Applied Immunology Research Center, Brasília - DF, 70910-900, Brazil.
Natalia I Z TiernoMaternal and Child Hospital of Brasilia Dr. Antonio Lisboa, Assisted Reproduction Sector, Brasilia-DF, 70203-900, Brazil.
Yamara A MacedoMaternal and Child Hospital of Brasilia Dr. Antonio Lisboa, Assisted Reproduction Sector, Brasilia-DF, 70203-900, Brazil.
Maria Eduarda B AmaralGenesis - Human Reproduction Assistance Center, Brasilia-DF, 70390-700, Brazil.
Hitomi M NakagawaGenesis - Human Reproduction Assistance Center, Brasilia-DF, 70390-700, Brazil.
Jair T GoulartUniversity of Brasilia, Institute of Biological Sciences, Department of Physiological Sciences, Brasilia-DF, 70910-900, Brazil.
Fernanda PauliniUniversity of Brasilia, Institute of Biological Sciences, Department of Physiological Sciences, Brasilia-DF, 70910-900, Brazil.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo examine the relationship between oxidative stress markers and reproductive parameters in women undergoing assisted reproductive treatments, focusing on the interaction between demographic, oxidative, and reproductive factors.

methodsA total of 49 patients with a mean age of 36.0±3.5years were evaluated. Biomarkers analyzed included reduced glutathione (GSH), disulfide glutathione (GSSG), total glutathione (tGSH), the GSSG/GSH ratio, reactive oxygen species (ROS), and reactive nitrogen species (RNS). Blood and follicular fluid samples were collected and analyzed, correlating these markers with variables such as antral follicle count (AFC), oocytes retrieved, mature oocytes, blastocyst formation, and pregnancy rates.

resultsThe results showed that AFC is a key marker of ovarian reserve, correlating positively with the number of oocytes retrieved and mature oocytes. Age significantly influenced antioxidant markers in both blood and follicular fluid, with a decline in antioxidant capacity linked to aging. In follicular fluid, higher GSSG levels correlated with the number of oocytes retrieved, indicating increased metabolic activity and ROS generation, thus raising oxidative burden during ovarian stimulation. Additionally, GSH and tGSH levels in blood negatively correlated with mature oocytes, suggesting greater antioxidant consumption during elevated metabolic demand.

conclusionsGlutathione is a robust and clinically promising redox biomarker for female fertility, evidencing strong correlations between systemic and follicular redox markers. Advancing age and repeated ovarian stimulation were shown to compromise antioxidant capacity, negatively impacting reproductive outcomes. Establishing reference values, particularly for the GSH/GSSG ratio, may enable its use as a prognostic tool, while the quantification of ROS and RNS could support personalized interventions and ovarian stimulation protocols.

Indexed as

Follicular FluidOvarian FollicleOvarian ReserveOxidative StressAdultBiomarkersFemaleGlutathioneHumansOocytesPregnancyPregnancy RateReactive Nitrogen SpeciesReactive Oxygen SpeciesBiomarkersGlutathioneReactive Nitrogen SpeciesReactive Oxygen Speciesassisted reproductionfemale fertilityoxidative biomarkersredox balancereproductive parameters

Identifiers

PMID41370417
PMCPMC12695009

What Socratic holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.