ArticleScientific reports2025
Comparative study of analytical methods for assessing mRNA integrity and identification of functional mRNA oligomers.
Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
2 citing papers in PubMed.
- From nucleotides to intact transcripts through oligonucleotides: Integrated chromatographic strategies for therapeutic mRNA characterization.Analytical and bioanalytical chemistry · 2026Review
- Analytical Characterization and Stability Assessment of RNA-Based Vaccines.Pharmaceutics · 2026Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Development of mRNA technologies has opened new horizons for both preventive vaccines and therapeutics. The rapid growth of mRNA products goes hand in hand with the need for novel analytical methods to characterize these products. One common critical quality attribute of mRNA Drug Substance (DS) and Drug Product (DP) is mRNA integrity. mRNA molecules are susceptible to degradation, often necessitating ultra-low-temperature storage of mRNA vaccines. Once degraded, mRNA can no longer be correctly translated into the target protein, leading to reduced vaccine efficacy. Therefore, robust analytical methods are crucial for understanding mRNA degradation mechanisms, optimizing formulation stability, and maximizing efficacy. In this study, we compared three analytical methods to assess mRNA integrity and purity: capillary gel electrophoresis (CGE) with fluorescent detection using a fragment analyzer (FA) device, CGE with ultraviolet (UV) detection, and ion-pairing reversed-phase chromatography (IP-RPLC) with UV detection. All three methods successfully monitored the integrity of mRNA DS and DP. IP-RPLC provided better resolution and facilitated the detection of mRNA-lipid adducts, while the FA, being more automated, enabled rapid analysis of a large number of samples. CGE-UV revealed the presence of mRNA oligomers. Further analysis showed that these oligomers could express the target protein similarly to the mRNA monomer.
Indexed as
Identifiers
What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.