ArticleFoods (Basel, Switzerland)2025
Development and Validation of an LC-MS/MS Method for Quantifying Phytohormones Related to Tomato Shelf Life.
Article in Foods (Basel, Switzerland), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Phytohormones are key signaling molecules that regulate plant growth, stress adaptation, and fruit ripening. However, their low abundance and structural diversity complicate accurate quantification in food matrices. This study presents a validated LC-MS/MS method for the simultaneous detection of seven phytohormones in tomato fruit, including two synthetic analogs that mimic natural auxins and cytokinins. Method optimization focused on extraction efficiency, solid-phase cleanup, and mobile phase composition, achieving high recovery (85-95%) and reduced matrix effects. Chromatographic separation was performed on a C18 column, with detection by triple quadrupole mass spectrometry in MRM mode. The method demonstrated excellent linearity (R
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