Evidence map›Paper›PMID 41376222›Full record

ArticleJournal of traditional Chinese medicine = Chung i tsa chih ying wen pan2025

Wenshen Zhuanggu Fang reduces breast cancer bone metastasis by regulating macrophage polarization: a bioinformatics analysis combined with experimental validation.

Zhang Yang, Shi Youyang, Liu Xiaofei, Yang Rui, L I Qiong, L I Feifei, Yang Xiaojuan, Wang Yi, Sun Chenping, Han Xianghui and 1 more

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Article in Journal of traditional Chinese medicine = Chung i tsa chih ying wen pan, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

11 authors.

Zhang YangInstitute of Chinese Traditional Surgery, Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China.
Shi YouyangInstitute of Chinese Traditional Surgery, Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China.
Liu XiaofeiDepartment of breast surgery, Affiliated Hospital of Shandong University of Traditional Chinese Medicine, Jinan 250014, China.
Yang RuiDepartment of breast surgery, Shanxi Provincial Cancer Hospital, Taiyuan 030000, China.
L I QiongDepartment of breast surgery, Yueyang Hospital of Traditional Chinese and Western Medicine Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200000, China.
L I FeifeiInstitute of Chinese Traditional Surgery, Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China.
Yang XiaojuanInstitute of Chinese Traditional Surgery, Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China.
Wang YiInstitute of Chinese Traditional Surgery, Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China.
Sun ChenpingInstitute of Chinese Traditional Surgery, Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China.
Han XianghuiInstitute of Chinese Traditional Surgery, Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China.
Liu ShengInstitute of Chinese Traditional Surgery, Longhua Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China.

Funding

Pharmacodynamic Substances and Mechanism of Wenshen Zhuanggu Recipe Regulating Exocrine Integrin/Sarcoma/Mitogen-activated protein kinase Signal Axis in Intervention of Bone Metastasis Niche Formation in Breast Cancer 82174016Scientific Connotation of Traditional Chinese Medicine "Conghua Theory" by Regulating Metastatic Site-specific Polarization of Macrophages in Breast Cancer Lung and Bone Metastases 81774308
6 · The paper itself

Abstract

objectiveTo explore the mechanism of Wenshen Zhuanggu Fang (, WSZG) against breast cancer bone metastasis from the perspective of macrophage polarization through bioinformatics and experiments.

methodsBioinformatics study was used to explore the mechanism underlying the effect of WSZG on breast cancer bone metastasis. Cell viability, migration, invasion and apoptosis assays were performed to detect the influence of WSZG on breast cancer cell MDA-MB-231BO promoted by macrophages. The protein expression level and cytokine content were detected by western blot and enzyme-linked immunosorbent assay kit

resultsM2 macrophages associated with poor prognosis and may lead to secondary bone metastasis in patients with triple-negative breast cancer. WSZG could treat breast cancer bone metastasis through regulating macrophage polarization by signal transducers and transcription signaling activators (STAT) signaling pathway. WSZG downregulated STAT6, CD206 and Arginase-1, while upregulated STAT1 and Inducible Nitric Oxide Synthase, thus inhibited the M2 macrophage-promoted invasion and migration capabilities of MDA-MB-231BO cells. WSZG treatment suppressed the bone metastasis of breast cancer, and the M2/M1 ratio was reduced by regulating STAT expression in bone metastatic tissue.

conclusionWSZG inhibited breast cancer bone metastasis by adjusting the promoting effect of macrophages on MDA-MB-231BO breast cancer cells and decreasing M2 polarization by downregulating STAT signaling.

Indexed as

Bone NeoplasmsBreast NeoplasmsDrugs, Chinese HerbalMacrophagesAnimalsCell Line, TumorCell MovementComputational BiologyFemaleHumansMiceSignal TransductionDrugs, Chinese Herbalbioinformatics studybreast cancer bone metastasismacrophage polarizationsignal transducers and transcription signaling activatorsWenshen Zhuanggu formula

Identifiers

PMID41376222
PMCPMC12711640

What Socratic holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.