ArticleTranslational cancer research2025
Developing of potential mRNA vaccines based on tumor antigens and immune subtypes of esophageal cancer.
Article in Translational cancer research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Background: Esophageal cancer (ESCA) carries a poor prognosis, and the exploration of mRNA vaccines for its treatment remains limited. This study aims to identify potential tumor antigens and characterize the immune landscape, thereby providing a foundation for developing mRNA vaccines against ESCA. Methods: A total of 150 and 179 specimens were analyzed using The Cancer Genome Atlas (TCGA)-ESCA and GSE53625 datasets. Quantitative real-time polymerase chain reaction (qRT-PCR) were performed on cDNA microarrays to verify the transcriptional levels of potential antigens. The immune subtypes were delineated using consensus clustering and module eigengenes were calculated by weighted gene co-expression network analysis (WGCNA). Results: We identified 5 tumor antigens with overexpression and mutation, which were associated with antigen-presentation and poor prognosis. A total of two subtypes (IS1 and IS2) were identified, and IS1 showed a better prognosis. The mutation count and tumor mutation burden were slightly higher, whereas immune checkpoint genes were lower in IS2. Compared with IS1, an increase in immune and stromal cell infiltration was associated with IS2, indicating that IS2 is immunologically "hot" and IS1 is immunologically "cold". Furthermore, ESCA patients' immune cell components were identified and survival outcome predictions were made by immune landscape construction. Immune hub genes, such as DKK1, could serve as biomarkers for predicting the prognosis and for vaccination. Finally, drug sensitivity analysis showed that IS1 patients might have higher sensitivity to TOP9 drugs with significant differences, which emphasized the importance of individualized treatment for ESCAs. Conclusions: We identified ANGPT2, CRIPT, GLA, LMNB1, and MARVELD3 as potential tumor antigens. Our study implies that IS2 phenotype might benefit from mRNA vaccination.
Indexed as
Identifiers
What Socratic holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.