Evidence map›Paper›PMID 41381303›Full record

ArticleAnnals of the rheumatic diseases2026

RUNX1 is expressed in a subpopulation of dermal fibroblasts and is associated with disease severity of systemic sclerosis.

Rezvan Parvizi, Zhiyun Gong, Helen C Jarnagin, Diana M Toledo, Tamar R Abel, Dillon Popovich, Madeline J Morrisson, Tammara A Wood, Sasha Shenk, Mrinal K Sarkar and 12 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Annals of the rheumatic diseases, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

22 authors.

Rezvan ParviziDepartment of Biomedical Data Science, Geisel School of Medicine at Dartmouth, Lebanon, NH, USA; Department of Molecular and Systems Biology, Geisel School of Medicine at Dartmouth, Hanover, NH, USA.
Zhiyun GongDepartment of Biomedical Data Science, Geisel School of Medicine at Dartmouth, Lebanon, NH, USA.
Helen C JarnaginDepartment of Biomedical Data Science, Geisel School of Medicine at Dartmouth, Lebanon, NH, USA; Department of Molecular and Systems Biology, Geisel School of Medicine at Dartmouth, Hanover, NH, USA.
Diana M ToledoDepartment of Biomedical Data Science, Geisel School of Medicine at Dartmouth, Lebanon, NH, USA; Department of Molecular and Systems Biology, Geisel School of Medicine at Dartmouth, Hanover, NH, USA.
Tamar R AbelDepartment of Biomedical Data Science, Geisel School of Medicine at Dartmouth, Lebanon, NH, USA; Department of Molecular and Systems Biology, Geisel School of Medicine at Dartmouth, Hanover, NH, USA.
Dillon PopovichDepartment of Biomedical Data Science, Geisel School of Medicine at Dartmouth, Lebanon, NH, USA; Department of Molecular and Systems Biology, Geisel School of Medicine at Dartmouth, Hanover, NH, USA.
Madeline J MorrissonDepartment of Biomedical Data Science, Geisel School of Medicine at Dartmouth, Lebanon, NH, USA; Department of Molecular and Systems Biology, Geisel School of Medicine at Dartmouth, Hanover, NH, USA.
Tammara A WoodDepartment of Biomedical Data Science, Geisel School of Medicine at Dartmouth, Lebanon, NH, USA.
Sasha ShenkDepartment of Basic and Clinical Translational Sciences, Tufts University School of Dental Medicine, Boston, MA, USA.
Mrinal K SarkarDepartment of Dermatology, University of Michigan, Ann Arbor, MI, USA.
Olesya PlazyoDepartment of Dermatology, University of Michigan, Ann Arbor, MI, USA.
Poulami DeyDepartment of Dermatology, University of Michigan, Ann Arbor, MI, USA; Division of Rheumatology, Department of Internal Medicine, University of Michigan, Ann Arbor, MI, USA.
Anthony CoonDepartment of Dermatology, University of Michigan, Ann Arbor, MI, USA.
Jake M ErbaDepartment of Dermatology, University of Michigan, Ann Arbor, MI, USA.
Lam C TsoiDepartment of Dermatology, University of Michigan, Ann Arbor, MI, USA.
Pei-Suen TsouDivision of Rheumatology, Department of Internal Medicine, University of Michigan, Ann Arbor, MI, USA.
Monique E HinchcliffSection of Rheumatology, Allergy and Immunology, Department of Medicine, Yale School of Medicine, New Haven, CT, USA.
Dinesh KhannaDivision of Rheumatology, Department of Internal Medicine, University of Michigan, Ann Arbor, MI, USA.
Jonathan A GarlickDepartment of Basic and Clinical Translational Sciences, Tufts University School of Dental Medicine, Boston, MA, USA.
Johann E GudjonssonDepartment of Dermatology, University of Michigan, Ann Arbor, MI, USA; Division of Rheumatology, Department of Internal Medicine, University of Michigan, Ann Arbor, MI, USA.
Patricia A PioliDepartment of Microbiology and Immunology, Geisel School of Medicine at Dartmouth, Lebanon, NH, USA.
Michael L WhitfieldDepartment of Biomedical Data Science, Geisel School of Medicine at Dartmouth, Lebanon, NH, USA; Department of Molecular and Systems Biology, Geisel School of Medicine at Dartmouth, Hanover, NH, USA. Electronic address: michael.whitfield@dartmouth.edu.

Funding

Deciphering the Mechanism of RUNX1 in Systemic Sclerosis (SSc) Dermal FibrosisF31AR084325 · NIAMS · DARTMOUTH COLLEGE · PI Rezvan Parvizi · 2025 to 2026
$99k
NIAMS NIH HHS F31 AR084325
6 · The paper itself

Abstract

objectivesThe activation of runt-related transcription factor 1 (RUNX1) in fibroblasts has been implicated in wound healing and fibrosis; however, the role of RUNX1 in the fibrotic progression of the autoimmune disease systemic sclerosis (SSc) remains known.

methodsLeveraging gene expression, genome-wide DNA methylation, and single-cell resolution data of SSc skin and fibroblast, we analysed the impact of RUNX1 dysregulation in SSc dermal fibrosis. RUNX1 function was subsequently assessed using siRNA, pharmacologic inhibition, and CRISPR knockout in 2-dimensional and 3-dimensional fibroblast cultures.

resultsAnalysis of gene expression in multiple cohorts demonstrated an association between the severity of dermal fibrosis and the expression levels of RUNX1 in the skin of patients with SSc. Epigenomic analyses of methylation identified hypomethylated 5-Cytosine-phosphate-Guanine-3 (CpG) sites proximal to the RUNX1 gene, implicating their potential role in the increased expression of RUNX1. Analysis of single-cell RNA-seq data from skin biopsies of individuals with SSc revealed that RUNX1 is higher in subpopulations of fibroblasts enriched in SSc, which are believed to contribute to fibrosis. RUNX1 CRISPR knockout resulted in reduced alpha smooth muscle actin expression. Inhibition of RUNX1 activity caused a reduction in fibroblast activation, contraction, extracellular matrix components, and proliferation rates, including a reduction in SFRP4, LUM, and COL1A1.

conclusionsThis study is the first to demonstrate a potential role for RUNX1 in the pathogenesis of SSc dermal fibrosis. RUNX1 is associated with more severe SSc fibrosis and is associated with a subpopulation of dermal fibroblasts implicated in fibrosis.

Indexed as

Core Binding Factor Alpha 2 SubunitFibroblastsScleroderma, SystemicSkinDNA MethylationFemaleFibrosisHumansMaleMiddle AgedSeverity of Illness IndexCore Binding Factor Alpha 2 SubunitRUNX1 protein, human

Identifiers

PMID41381303
PMCPMC12969718

What Socratic holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.