Evidence map›Paper›PMID 41388511›Full record

ArticleBMC genomics2025

D-limonene inhibits the growth of Fusarium proliferatum by decreasing H3K9ac and H3K27ac modifications.

Shi-Wei Zhou, Yao Zhu, Xue-Jie Qin, You Li, Sulukkana Noiprasert, Kanyaphat Apiwongsrichai, Fu-Rong Xu, Xiao-Yun Liu, Xian Dong

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC genomics, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Shi-Wei ZhouYunnan Key Laboratory of Chinese Medicine Processing, College of Chinese Materia Medica, Yunnan University of Chinese Medicine, Kunming, China.
Yao ZhuYunnan Key Laboratory of Chinese Medicine Processing, College of Chinese Materia Medica, Yunnan University of Chinese Medicine, Kunming, China.
Xue-Jie QinYunnan Key Laboratory of Chinese Medicine Processing, College of Chinese Materia Medica, Yunnan University of Chinese Medicine, Kunming, China.
You LiYunnan Key Laboratory of Chinese Medicine Processing, College of Chinese Materia Medica, Yunnan University of Chinese Medicine, Kunming, China.
Sulukkana NoiprasertSchool of Integrative Medicine, Mae Fah Luang University, 333 Moo 1, Thasud, Muang, Chiang Rai, 57100, Thailand.
Kanyaphat ApiwongsrichaiSchool of Integrative Medicine, Mae Fah Luang University, 333 Moo 1, Thasud, Muang, Chiang Rai, 57100, Thailand.
Fu-Rong XuYunnan Key Laboratory of Chinese Medicine Processing, College of Chinese Materia Medica, Yunnan University of Chinese Medicine, Kunming, China.
Xiao-Yun LiuHanjiang River Basin/Jianghan University, Wuhan, 43005, China. liuxiaoyun@jhun.edu.cn.
Xian DongYunnan Key Laboratory of Chinese Medicine Processing, College of Chinese Materia Medica, Yunnan University of Chinese Medicine, Kunming, China. dongxian_1655129@163.com.

Funding

2025 Yunnan Provincial International Science and Technology Commissioner (Individual-Based) 202503AK140002Natural Science Foundation of Hubei Province 2023AFB982Wang Yuan Chao Expert Workstation in Yunnan Province 202305AF150018Yunnan International Joint Laboratory for Exploration and Utilization of Aromatic Chinese Medicinal Materials in Agriculture 202503AP140024Yunnan Provincial Science and Technology Plan-Basic Research Project 202301AW070008
6 · The paper itself

Abstract

backgroundFusarium proliferatum, a major pathogen causing root rot in Panax notoginseng, severely threatens sustainable cultivation. While chemical pesticides face resistance and environmental risks, plant-derived alternatives are urgently needed. D-limonene, a natural monoterpene with broad antimicrobial activity, remains unexplored against F. proliferatum.

resultsD-limonene strongly inhibited F. proliferatum growth: 200 mM suppressed hyphal growth by 73.89%, reduced sporulation by 73.6%, and impaired conidia germination. It disrupted membrane integrity, increasing extracellular conductivity by 64.3% within 4 h. Transcriptomics revealed 1,134 upregulated and 1,240 downregulated genes, with downregulated genes enriched in ribosomal translation and endoplasmic reticulum protein processing. Critically, D-limonene reduced histone acetylation (H3K9ac/H3K27ac). ChIP-seq confirmed that H3K9 hypoacetylation inhibited ribosomal genes (RPS9, RPS3, RPS4), while H3K27 hypoacetylation suppressed lipid metabolism and mitochondrial biogenesis, collectively stalling fungal growth.

conclusionsD-limonene combats F. proliferatum by damaging cell membranes and reprogramming histone acetylation to disrupt ribosome function and mitochondrial biosynthesis. This dual-action mechanism highlights its potential as an eco-friendly botanical fungicide against root rot.

Indexed as

FusariumHistonesLimoneneAcetylationGene Expression Regulation, FungalHistonesLimoneneChIP-seqLipid metabolismPlant-derived pesticidesRibosome structure compositionRNA-seq

Identifiers

PMID41388511
PMCPMC12817427

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.