Shaoyao gancao decoction induces autophagy by modulating miR-21 and inhibiting the AKT/mTOR signaling pathway in adenomyosis-derived ectopic endometrial stromal cells.

Ethnopharmacological relevance: Shaoyao Gancao Decoction (SGD) is a renowned traditional Chinese medicinal formulation widely utilized in Asia. It is particularly effective in treating spastic, painful, and inflammatory conditions, especially gynecological pain disorders. However, the underlying mechanisms of its action remain largely unexplored. Aims of the study: To investigate the potential molecular mechanism by which SGD inhibits autophagy and apoptosis in ectopic endometrial stromal cells (EESCs), thereby elucidating the pathway responsible for its anti-adenomyosis effects. Materials and methods: Four types of tissues were collected for detection, including AM ectopic endometrium, AM eutopic endometrium, normal myometrium, and normal endometrium. Meanwhile, EESCs were cultured Results: In ectopic endometria of AM, there was a significant increase in miR-21 mRNA expression as well as heightened activity within the phosphatidylinositol 3-kinase/protein kinase B/mammalian target of rapamycin (PI3K/AKT/mTOR) signaling pathway. Conversely, PTEN expression was found to be decreased. Additionally, autophagy-related protein levels were diminished while anti-apoptotic protein expression was markedly elevated ( Conclusion: The progression of AM is associated with autophagy inhibition in ectopic lesions. SGD can exert autophagy-promoting and pro-apoptotic effects in EESCs, and this process occurs by inhibiting the AKT/mTOR signaling pathway with miR-21 as the target. These findings unveil the potential novel molecular mechanisms from the perspective of microRNA by which SGD suppresses the growth of EESCs.