Evidence map›Paper›PMID 41395255›Full record

ArticleResearch (Washington, D.C.)2025

An Integrated Electrolysis-Enrichment Microchip for Ultra-Rapid and Sensitive mRNA Detection.

Long Cheng, Zhiying Wang, Chengbao Wu, Feng Liu, Hui Li, Yunke Feng, Xi Chen, Xinxin Hang, Yu Zeng, Wei Mu and 6 more

Abstract read
In one paragraph

Article in Research (Washington, D.C.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Long ChengSchool of Biomedical Engineering, Anhui Medical University, Hefei 230032, China.
Zhiying WangKey Laboratory of Biomechanics and Mechanobiology, Ministry of Education, Beijing Advanced Innovation Center for Biomedical Engineering, Beihang University, Beijing 100191, China.
Chengbao WuSchool of Biomedical Engineering, Anhui Medical University, Hefei 230032, China.
Feng LiuKey Laboratory of Biomechanics and Mechanobiology, Ministry of Education, Beijing Advanced Innovation Center for Biomedical Engineering, Beihang University, Beijing 100191, China.
Hui LiDepartment of Oncology, the Fifth Medical Center, Chinese PLA General Hospital, Beijing 100071, China.
Yunke FengDepartment of Oncology, the Fifth Medical Center, Chinese PLA General Hospital, Beijing 100071, China.
Xi ChenDepartment of Ophthalmology, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China.
Xinxin HangKey Laboratory of Biomechanics and Mechanobiology, Ministry of Education, Beijing Advanced Innovation Center for Biomedical Engineering, Beihang University, Beijing 100191, China.
Yu ZengKey Laboratory of Biomechanics and Mechanobiology, Ministry of Education, Beijing Advanced Innovation Center for Biomedical Engineering, Beihang University, Beijing 100191, China.
Wei MuKey Laboratory of Biomechanics and Mechanobiology, Ministry of Education, Beijing Advanced Innovation Center for Biomedical Engineering, Beihang University, Beijing 100191, China.
Yuhao ZhouKey Laboratory of Biomechanics and Mechanobiology, Ministry of Education, Beijing Advanced Innovation Center for Biomedical Engineering, Beihang University, Beijing 100191, China.
Liye LiuSchool of Biomedical Engineering, Anhui Medical University, Hefei 230032, China.
Lingqian ChangSchool of Biomedical Engineering, Anhui Medical University, Hefei 230032, China.
Qiaowei LiuDepartment of Oncology, the Fifth Medical Center, Chinese PLA General Hospital, Beijing 100071, China.
Yi HuDepartment of Oncology, the Fifth Medical Center, Chinese PLA General Hospital, Beijing 100071, China.
Yang WangKey Laboratory of Biomechanics and Mechanobiology, Ministry of Education, Beijing Advanced Innovation Center for Biomedical Engineering, Beihang University, Beijing 100191, China.ORCID https://orcid.org/0009-0009-3913-2442

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Messenger RNA (mRNA) detection plays a vital role in gene expression analysis, disease diagnosis, and precision medicine. However, current methods are limited by complex chemical-based extraction procedures and the rapid degradation of mRNA. To overcome these challenges, we developed an electrolysis-enrichment microfluidic chip (EEMC) that employs electricity-driven physical methods for ultra-rapid, efficient mRNA extraction directly from whole blood, eliminating the need for chemical reagents that can inhibit downstream analysis. By integrating electrolytic lysis with ion concentration polarization (ICP) enrichment, the EEMC completes the entire process within 10 min-6 times faster than conventional techniques, greatly avoiding the potential degradation. It achieves a high recovery efficiency of 90% to 95%, which surpasses the 85% to 90% efficiency of commercial RNA extraction kits. Combined with downstream loop-mediated isothermal amplification (LAMP), the EEMC provides a powerful solution for rapid mRNA processing and detection. Its efficacy was validated in radiation exposure models through the detection of key mRNA biomarkers (BAX, CDKN1A, and GADD45A). This environmentally friendly and high-performance platform advances point-of-care precision diagnostics.

Identifiers

PMID41395255
PMCPMC12696694

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.