Evidence map›Paper›PMID 41400846›Full record

ArticleBiogerontology2025

Effect of Hong Huang Tang on memory enhancement and mitigation of microgravity-induced oxidative stress in C. elegans.

Inam Ullah, Muhammad Zulqarnain Shakir, Xu Chen Zhou, Jixian Liu, Shilan Li, Huabiao Chen, Ning Jiang, Muhammad Wasim Usmani, Muhammad Qasim Barkat, Qiaobei Du and 3 more

Abstract read
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In one paragraph

Article in Biogerontology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Inam UllahInstitute of Drug Discovery Technology, Ningbo University, Ningbo, 315211, China.
Muhammad Zulqarnain ShakirInstitute of Drug Discovery Technology, Ningbo University, Ningbo, 315211, China.
Xu Chen ZhouInstitute of Drug Discovery Technology, Ningbo University, Ningbo, 315211, China.
Jixian LiuInstitute of Drug Discovery Technology, Ningbo University, Ningbo, 315211, China.
Shilan LiInstitute of Drug Discovery Technology, Ningbo University, Ningbo, 315211, China.
Huabiao ChenHealth BioMed Co., Ltd., Ningbo, 315040, Zhejiang, China.
Ning JiangResearch Center for Pharmacology and Toxicology, Institute of Medicinal Plant Development (IMPLAD), Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100050, China.
Muhammad Wasim UsmaniInstitute of Drug Discovery Technology, Ningbo University, Ningbo, 315211, China.
Muhammad Qasim BarkatInstitute of Drug Discovery Technology, Ningbo University, Ningbo, 315211, China.
Qiaobei DuInstitute of Drug Discovery Technology, Ningbo University, Ningbo, 315211, China.
Yufen ZhaoInstitute of Drug Discovery Technology, Ningbo University, Ningbo, 315211, China.
Ning WangInstitute of Drug Discovery Technology, Ningbo University, Ningbo, 315211, China. wangning2@nbu.edu.cn.
Xinmin LiuInstitute of Drug Discovery Technology, Ningbo University, Ningbo, 315211, China. liuxinmin@hotmail.com.

Funding

Major Science and Technology Project in the Autonomous Region 2023A02010-3National Natural Science Foundation of China 42388101, 92256203Ningbo Top Talent Project 215-432094250Space Medical Experiment Project of CMSP HYZ1XK02003The Health Fund of Translational Biomedicine H2024000279
6 · The paper itself

Abstract

Ziziphus jujuba Mill. (ZJ) is a traditional medicinal plant known for its antioxidant, anti-inflammatory, and neuroprotective properties, yet its role in learning and cognitive regulation remains insufficiently explored. Huang Jing (Polygonatum sibiricum), a Qi- and Yin-tonifying herb in Traditional Chinese Medicine, has historically been used to combat fatigue, support brain function, delay aging, and regulate metabolic balance. In this study, we evaluated the neuroprotective and antioxidant effects of the combined formulation Hong Huang Tang in Caenorhabditis elegans under simulated microgravity conditions. Behavioral assays, including lifespan, chemotaxis-based learning, pharyngeal pumping, head thrashing, and body bending, were performed to assess cognitive and neuromuscular function. Mitochondrial health and oxidative stress markers were quantified, alongside expression of antioxidant defense genes. DAF-16::GFP localization and sod-3 expression were analyzed to determine involvement of insulin/IGF-1 signaling. Additionally, neuroprotection against 6-hydroxydopamine-induced dopaminergic degeneration was assessed. Simulated microgravity triggered oxidative stress, mitochondrial dysfunction, reduced lifespan, impaired learning, and neuromuscular decline. Treatment with 2 mg/mL Hong Huang Tang significantly reversed these effects, restoring mitochondrial function, enhancing antioxidant capacity, and alleviating neurodegeneration. These findings support Hong Huang Tang as a promising therapeutic candidate for oxidative stress-related cognitive decline and neurodegenerative disorders.

Indexed as

Caenorhabditis elegansDrugs, Chinese HerbalMemoryNeuroprotective AgentsOxidative StressWeightlessnessAnimalsAntioxidantsCaenorhabditis elegans ProteinsLongevityMitochondriaWeightlessness SimulationAntioxidantsCaenorhabditis elegans ProteinsDrugs, Chinese HerbalNeuroprotective AgentsAntioxidant activityCaenorhabditis elegansDAF-16/insulin signallingHuang JingLearning and memoryMitochondrial dysfunctionNeuroprotectionSimulated microgravityZiziphus jujuba Mill.

Identifiers

PMID41400846

What Socratic holds

Textmetadata
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.