ArticleBrain and behavior2025
Electroacupuncture Potentiates Astragaloside IV Cerebral Delivery via P-Glycoprotein-Mediated Transcellular Transport: A Novel Blood-Brain Barrier Penetration Strategy for Ischemic Stroke Therapy.
Article in Brain and behavior, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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Who cites it
3 citing papers in PubMed.
- Transcriptomic Insights into Acupuncture Mechanisms in Protecting Ovarian Function in Mice with Premature Ovarian Insufficiency.Chinese journal of integrative medicine · 2026Article
- Electroacupuncture enables semaglutide dose sparing in type 2 diabetes by boosting drug concentration and suppressing β-cell apoptosis.Frontiers in endocrinology · 2026Article
- Electroacupuncture Potentiates Astragaloside IV Cerebral Delivery via P-Glycoprotein-Mediated Transcellular Transport: A Novel Blood-Brain Barrier Penetration Strategy for Ischemic Stroke Therapy.Brain and behavior · 2025Article
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15 authors.
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Abstract
backgroundAstragaloside IV (AS-IV) exhibits therapeutic potential in central nervous system (CNS) disorders. However, its brain bioavailability is restricted by P-glycoprotein (P-gp)-mediated efflux at the blood-brain barrier (BBB). This study aims to investigate whether electroacupuncture (EA) can enhance the cerebral delivery of AS-IV by inhibiting nuclear factor-kappa B (NF-κB) nuclear translocation to downregulate P-gp expression.
methodsThe permeability of the BBB was assessed using Evans blue (EB) staining and transmission electron microscopy. The intracerebral concentration of AS-IV was quantified by liquid chromatography-tandem mass spectrometry (LC-MS/MS). The therapeutic efficacy of AS-IV in combination with EA was evaluated using triphenyltetrazolium chloride (TTC) staining and neurological function assessments. mRNA and protein expression levels of relevant factors were analyzed through real-time quantitative polymerase chain reaction (RT-qPCR), western blot, and immunofluorescence.
resultsIn normal mice, EA effectively increases the levels of EB and AS-IV in brain tissue. The combination of EA and AS-IV significantly activates the Wnt/β-catenin signaling pathway and promotes the expression of zonula occludens protein (ZO)-1 and Claudin-5. Furthermore, EA inhibits NF-κB nuclear translocation and mitigates AS-IV-induced upregulation of P-gp expression. Notably, EA significantly elevates the AS-IV content in ischemic brain tissue. The combination therapy demonstrates enhanced therapeutic effects in ischemic rats, including reductions in neurological function scores, infarct size, and pro-inflammatory factor levels, along with increased expression of anti-inflammatory factors. Additionally, this combination significantly inhibits NF-κB nuclear translocation and reduces P-gp expression in the brain tissue of ischemic stroke rats.
conclusionThese findings indicate that EA enhances the brain uptake and neuroprotective effects of AS-IV, irrespective of BBB integrity. The underlying mechanism involves P-gp-mediated transcellular transport rather than the paracellular pathway.
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