Evidence map›Paper›PMID 41405182›Full record

ArticleBrain and behavior2025

Neuroprotective Effects of Tuina in CP Rats Are Associated With Gut Microbiota Remodeling and Intestinal Barrier Restoration.

Chenqin Si, Rui Qiao, Yu Liu, Ayipaxiaguli Kasimu, Danmei Chen, Lijian Xie, Ping-Ping Sun, Bing Li

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PubMed Full text at PMC Publisher
In one paragraph

Article in Brain and behavior, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Chenqin SiDepartment of Traditional Chinese Medicine, Jinshan Hospital Affiliated to Fudan University, Shanghai, China.ORCID https://orcid.org/0000-0002-3036-0440
Rui QiaoResearch Center for Clinical Medicine, Jinshan Hospital Affiliated to Fudan University, Shanghai, China.ORCID https://orcid.org/0000-0001-9366-6503
Yu LiuDepartment of Traditional Chinese Medicine, Jinshan Hospital Affiliated to Fudan University, Shanghai, China.ORCID https://orcid.org/0009-0000-1312-9354
Ayipaxiaguli KasimuDepartment of Traditional Chinese Medicine, Jinshan Hospital Affiliated to Fudan University, Shanghai, China.ORCID https://orcid.org/0000-0001-9382-3011
Danmei ChenDepartment of Traditional Chinese Medicine, Jinshan Hospital Affiliated to Fudan University, Shanghai, China.ORCID https://orcid.org/0000-0002-7065-9922
Lijian XieDepartment of Pediatrics, Jinshan Hospital, Affiliated to Fudan University, Shanghai, China.ORCID https://orcid.org/0000-0002-0328-6820
Ping-Ping SunSchool of Rehabilitation Science, Shanghai University of Traditional Chinese Medicine, Shanghai, China.ORCID https://orcid.org/0000-0002-8446-5598
Bing LiDepartment of Traditional Chinese Medicine, Jinshan Hospital Affiliated to Fudan University, Shanghai, China.ORCID https://orcid.org/0000-0001-5709-9396

Funding

Medical Key Disciplines of Shanghai 2024ZDXK0056National Natural Science Foundation of China 82174522National Natural Science Foundation of China 82305428Seventh Period Jinyin Medical Key Specialty A-level Pediatrics JSZK2023A04
6 · The paper itself

Abstract

introductionCerebral palsy (CP) is a neurodevelopmental disorder that has been linked to gut microbiota dysbiosis. Although Tuina has shown neuroprotective effects, it remains unclear whether these benefits involve regulation of the gut-brain axis. This study aimed to evaluate the therapeutic effects of Tuina in CP rats, with emphasis on its potential regulation of the gut-brain axis.

methodsCP was induced in 7-day-old Sprague-Dawley rats through hypoxia-ischemia. Beginning on postnatal day 8 (P8), the Tuina group received daily Tuina therapy for 32 consecutive days. Motor function was assessed using the negative geotaxis test (P6-P12), the beam balance test (P36-P39), and the modified neurological severity score on P40. Gut microbiota composition was analyzed using 16S rRNA sequencing. Brain and intestinal histopathology were evaluated histologically via hematoxylin-eosin and Luxol fast blue staining. Protein expression of BDNF, Nrf2, GPX4, ZO-1, and occludin was assessed via western blotting and immunofluorescence. Serum short-chain fatty acids (SCFAs) were measured by mass spectrometry, whereas oxidative stress and intestinal barrier markers (superoxide dismutase, malondialdehyde, glutathione peroxidase, lipopolysaccharide [LPS], diamine oxidase [DAO], and D-lactate [D-LA]) were detected using enzyme-linked immunosorbent assay.

resultsIn CP models induced by hypoxic-ischemic encephalopathy, significant brain injury and motor dysfunction were observed, accompanied by gut microbiota dysbiosis and impaired intestinal barrier function. Tuina intervention improved motor function and growth, regulated gut microbiota, and increased serum SCFA levels. It also enhanced intestinal barrier proteins (occludin, ZO-1), reduced serum levels of LPS, DAO, and D-LA, and increased the expression of brain-derived BDNF, Nrf2, and GPX4.

conclusionTuina significantly alleviated brain injury and improved motor function in CP rats. These effects were associated with modulation of the gut microbiota and restoration of intestinal barrier integrity, suggesting that the gut-brain axis may mediate the neuroprotective effects of Tuina.

Indexed as

Cerebral PalsyGastrointestinal MicrobiomeNeuroprotective AgentsAnimalsBrainDisease Models, AnimalDysbiosisMaleOxidative StressRatsRats, Sprague-DawleyNeuroprotective Agentscerebral palsygut–brain axisgut microbiotaintestinal barrier

Identifiers

PMID41405182
PMCPMC12710083

What Socratic holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.