Evidence map›Paper›PMID 41405660›Full record

ArticleVeterinary research communications2025

Synchronous natural infection of Nile tilapia (Oreochromis niloticus) by drug-resistant Pseudomonas aeruginosa and Saprolegnia parasitica: insights into water quality, phenotypic traits, and genotypic profiles.

Reham M El-Tarabili, Mahmoud E Elsayed, Hanan S Khalefa, Hanan Elghayaty, Zainab Mohamed El Kattawy, Mohamed Marzok, Sherief M Abdel-Raheem, Hesham L Ismail, Dalia A Abdel-Moneam

Abstract read
PubMed Publisher
In one paragraph

Article in Veterinary research communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Reham M El-TarabiliDepartment of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, 41522, Egypt. rehameltrabely@gmail.com.ORCID http://orcid.org/0000-0001-8273-2747
Mahmoud E ElsayedDepartment of Bacteriology, Immunology and Mycology, Faculty of Veterinary Medicine, Suez Canal University, Ismailia, 41522, Egypt.
Hanan S KhalefaDepartment of Veterinary Hygiene and Management, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, Egypt.
Hanan ElghayatyPortsaid laboratory Branch, Animal Health Research Institute, Portsaid, Egypt.
Zainab Mohamed El KattawyPortsaid laboratory Branch, Animal Health Research Institute, Portsaid, Egypt.
Mohamed MarzokDepartment of Clinical Sciences, College of Veterinary Medicine, King Faisal University, P.O. Box 400, 31982, Al-Ahsa, Hofuf, Saudi Arabia.
Sherief M Abdel-RaheemDepartment of Public Health, College of Veterinary Medicine, King Faisal University, P.O. Box 400, Al-Hofuf, Al-Ahsa, 31982, Saudi Arabia.
Hesham L IsmailDepartment of Public Health, College of Veterinary Medicine, King Faisal University, P.O. Box 400, Al-Hofuf, Al-Ahsa, 31982, Saudi Arabia. hismail@kfu.edu.sa.
Dalia A Abdel-MoneamDepartment of Aquatic Animal Medicine and Management, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, Egypt.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

This study investigated the concurrent infection of Pseudomonas aeruginosa and Saprolegnia parasitica in Oreochromis niloticus from Lake Manzala during the period from October to December 2023, employing phenotypic and genotypic characterizations of the isolated pathogens. Naturally infected fish displayed septicemic picture, fin rot, and the presence of cotton wool-like masses on the skin and fins. Water quality analysis revealed elevated salinity, unionized ammonia and nitrite levels, alongside with suboptimal water temperature. P. aeruginosa and S. parasitica were isolated from 30% and 31.8% of the clinically affected and moribund examined fish, respectively. Molecular analysis of P. aeruginosa 16 S-rDNA and S. parasitica ITS-rDNA genes demonstrated a genetic similarity with their ancestral strains from the families Pseudomonadaceae and Saprolegniaceae, respectively. The pathogenicity of P. aeruginosa was confirmed through β-hemolysis and biofilm production. Antimicrobial susceptibility testing of P. aeruginosa indicated high susceptibility to colistin sulfate (100%) and meropenem (87.95%), with notable resistance to tetracycline (100%) and ampicillin (93.9%). Virulence gene analysis identified several key genes, including toxA (93.9%), exoS (66.7%), lasB (45.45%), phzM (33.3%), pslA (33.3%), and fliC (42.4%). Resistance profiling revealed the presence of multiple antibiotic resistance genes, including tetA (100%), bla

Indexed as

CichlidsFish DiseasesPseudomonas aeruginosaPseudomonas InfectionsSaprolegniaAnimalsAnti-Bacterial AgentsDrug Resistance, BacterialDrug Resistance, Multiple, BacterialGenotypePhenotypeWater QualityAnti-Bacterial AgentsAntimicrobial resistanceConcurrent infectionOreochromis niloticusPseudomonas aeruginosaSaprolegnia parasitica

Identifiers

PMID41405660

What Socratic holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.