Evidence map›Paper›PMID 41407940›Full record

ArticleThe AAPS journal2025

Sodium Alginate Hydrogels Loaded with Mesenchymal Stem Cells-Derived Extracellular Vesicles: Safety and Cell Migration Potential.

Lucas Henrique Domingos da Silva, José Alberto Paris Junior, Rauany Cristina Lopes Francisco, Eliane Trovatti, Alberto Gomes Tavares Junior, Marlus Chorilli, Felipe Falcão Haddad, Cauê Benito Scarim, Juliana Ferreira Floriano, Pedro Fardim and 1 more

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Article in The AAPS journal, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Lucas Henrique Domingos da SilvaUniversidade de Araraquara - UNIARA, Carlos Gomes 1338, 14801-340, Araraquara, São Paulo, Brazil.ORCID 0000-0002-7505-7568
José Alberto Paris JuniorUniversidade de Araraquara - UNIARA, Carlos Gomes 1338, 14801-340, Araraquara, São Paulo, Brazil.ORCID 0000-0003-2312-3866
Rauany Cristina Lopes FranciscoUniversidade de Araraquara - UNIARA, Carlos Gomes 1338, 14801-340, Araraquara, São Paulo, Brazil.ORCID 0000-0003-4121-8797
Eliane TrovattiUniversidade de Araraquara - UNIARA, Carlos Gomes 1338, 14801-340, Araraquara, São Paulo, Brazil.ORCID 0000-0002-0495-8115
Alberto Gomes Tavares JuniorUniversidade do Estado de São Paulo - UNESP, Faculdade de Ciências Farmacêuticas, Araraquara, São Paulo, Brazil.ORCID 0000-0002-6083-8177
Marlus ChorilliUniversidade do Estado de São Paulo - UNESP, Faculdade de Ciências Farmacêuticas, Araraquara, São Paulo, Brazil.ORCID 0000-0002-6698-0545
Felipe Falcão HaddadUniversidade do Estado de São Paulo - UNESP, Faculdade de Ciências Farmacêuticas, Araraquara, São Paulo, Brazil.ORCID 0009-0005-1772-7956
Cauê Benito ScarimUniversidade do Estado de São Paulo - UNESP, Faculdade de Ciências Farmacêuticas, Araraquara, São Paulo, Brazil.ORCID 0000-0002-2540-6395
Juliana Ferreira FlorianoNational Heart and Lung Institute, Imperial College London, London, United Kingdom.ORCID 0000-0002-7423-7591
Pedro FardimKatholieke Universiteit Leuven, Chemical and Biochemical Reactor Engineering and Safety (CREaS), Louvain, Belgium.ORCID 0000-0003-1545-3523
Flávia Aparecida ResendeUniversidade de Araraquara - UNIARA, Carlos Gomes 1338, 14801-340, Araraquara, São Paulo, Brazil. farnogueira@uniara.edu.br.ORCID 0000-0002-9432-5919

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Wound healing is a complex process often impaired in severe injuries, requiring innovative therapeutic strategies. Mesenchymal stem cell-derived extracellular vesicles (MSC-EVs) modulate key cellular pathways, but their clinical application is limited by low stability and bioavailability. This study aimed to evaluate the safety and potential of sodium alginate hydrogels (SAH-EVs) loaded with mesenchymal stem cell-derived extracellular vesicles, focusing on cell migration, cytotoxicity, genotoxicity, and irritation potential. MSC-EVs from Sprague-Dawley rat bone marrow were isolated from conditioned medium collected at 24, 36, 48 and 60 h using size exclusion chromatography and characterized by Nanoparticle Tracking Analysis. The highest EV concentration was obtained from the conditioned medium collected at 36 h, with a main peak at 123 nm. The heterogeneous particle population suggests the presence of EV subtypes. Scanning Electron Microscopy confirmed successful MSC-EVs incorporation into hydrogels with desirable viscoelastic properties. SAH-EVs stimulated HaCaT keratinocyte migration while exhibiting low cytotoxicity in 2D and 3D models, with no genotoxic or mutagenic effects. HET-CAM assays confirmed the absence of irritation potential. These findings highlight the potential of SAH-EVs as a safe biomaterial and lay the groundwork for further investigations into their role in wound healing, reinforcing their relevance in regenerative medicine and tissue engineering.

Indexed as

AlginatesCell MovementExtracellular VesiclesHydrogelsMesenchymal Stem CellsAnimalsCell LineCell SurvivalGlucuronic AcidHexuronic AcidsHumansRatsRats, Sprague-DawleyWound HealingAlginatesGlucuronic AcidHexuronic AcidsHydrogelscell migrationcyto-genotoxicitymesenchymal stem cells-derived extracellular vesiclessodium alginate hydrogels

Identifiers

PMID41407940

What Socratic holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.