ArticlebioRxiv : the preprint server for biology2025
Bioenergetic responses to β-adrenergic stimulation in beige adipocyte depend on actomyosin driven forces.
Yuchen He, Lu Ling, Garrett Dempsey, Nayiri Kalindjian, Zoie Verghese, Erzhen Chen, Irene Liparulo, Isabel Cho, Vyvylyn Tran, Sanjay Kumar and 1 more
Abstract readPreprint
In one paragraphArticle in bioRxiv : the preprint server for biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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0citing papers in PubMed
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What it found
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2 · The registryThe trial behind it
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3 · Its place in the literatureWho cites it
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4 · The recordCorrections and comments
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5 · Who and what moneyAuthors and funding
11 authors.
Yuchen HeDepartment of Nutritional Science and Toxicology, College of Natural Resources, University of California Berkeley, Berkeley, California, 94720, USA.ORCID 0009-0006-5217-9091 Lu LingDepartment of Bioengineering, University of California Berkeley, Berkeley, California, 94720, USA.ORCID 0000-0001-6693-8665 Garrett DempseyDepartment of Nutritional Science and Toxicology, College of Natural Resources, University of California Berkeley, Berkeley, California, 94720, USA.
Nayiri KalindjianDepartment of Nutritional Science and Toxicology, College of Natural Resources, University of California Berkeley, Berkeley, California, 94720, USA.
Zoie VergheseDepartment of Nutritional Science and Toxicology, College of Natural Resources, University of California Berkeley, Berkeley, California, 94720, USA.
Erzhen ChenDepartment of Nutritional Science and Toxicology, College of Natural Resources, University of California Berkeley, Berkeley, California, 94720, USA.ORCID 0009-0003-5829-1509 Irene LiparuloDepartment of Nutritional Science and Toxicology, College of Natural Resources, University of California Berkeley, Berkeley, California, 94720, USA.ORCID 0000-0002-2069-0067 Isabel ChoDepartment of Nutritional Science and Toxicology, College of Natural Resources, University of California Berkeley, Berkeley, California, 94720, USA.ORCID 0009-0000-9292-0821 Vyvylyn TranDepartment of Nutritional Science and Toxicology, College of Natural Resources, University of California Berkeley, Berkeley, California, 94720, USA.
Sanjay KumarDepartment of Bioengineering, University of California Berkeley, Berkeley, California, 94720, USA.ORCID 0000-0002-9996-4883 Andreas StahlDepartment of Nutritional Science and Toxicology, College of Natural Resources, University of California Berkeley, Berkeley, California, 94720, USA.ORCID 0000-0002-9096-3024 Funding
Cellular mechanobiology and engineering of active brown adipose tissueR01DK118940 · NIDDK · UNIVERSITY OF CALIFORNIA BERKELEY · PI KUMAR, SANJAY, STAHL, ANDREAS · 2019 to 2022
$2.3MRole of Biomechanical Interfaces Created by Focal Adhesion Kinase in Catecholamine SignalingR01DK141135 · NIDDK · UNIVERSITY OF CALIFORNIA BERKELEY · PI Sanjay Kumar, Andreas Stahl · 2024 to 2026
$2.0MICP-QQQS10OD030258 · OD · UNIVERSITY OF CALIFORNIA BERKELEY · PI JORGENS, DANIELLE MARY · 2021 to 2021
$1.6MAutomated High-Throughput, High-Content Confocal/Wide-Field Microscope SystemS10OD021828 · OD · UNIVERSITY OF CALIFORNIA BERKELEY · PI WEST, MARY · 2017 to 2017
$1.0MUpgrade for time-correlated single photon counting fluorescence lifetime laser scanning confocal microscopeS10OD025063 · OD · UNIVERSITY OF CALIFORNIA BERKELEY · PI AARON, HOLLY L · 2019 to 2019
$862kA Zeiss Elyra S1 Structured Illumination Microscope for the Biological Imaging FaS10OD018136 · OD · UNIVERSITY OF CALIFORNIA BERKELEY · PI RUZIN, STEVEN EARL · 2014 to 2014
$600kA Zeiss LSM 710 confocal microscope for the Biological Imaging Facility at the US10RR026866 · NCRR · UNIVERSITY OF CALIFORNIA BERKELEY · PI RUZIN, STEVEN EARL · 2010 to 2010
$419kNCRR NIH HHS S10 RR026866NIDDK NIH HHS R01 DK118940NIDDK NIH HHS R01 DK141135NIH HHS S10 OD018136NIH HHS S10 OD021828NIH HHS S10 OD025063NIH HHS S10 OD030258
6 · The paper itselfAbstract
Beige (BeAT) and white adipoctyes (WAT) reside within the same fat depots but differ in their responses to β-adrenergic stimulation. While both activate classical PKA-mediated pathways, only beige adipocytes show induction of UCP1 expression and enhanced mitochondrial respiration, suggesting additional mechanisms that distinguish their responses. Here we uncover a parallel, non-canonical biomechanical pathway specific to beige adipocytes that is essential for thermogenic activation. Mechanistically, β-AR stimulation induces a rapid Myh9-mediated actomyosin contraction and stiffening in beige, but not white, adipocytes that activates focal adhesion kinase (FAK), which we show to be critical for the expression of oxidative and thermogenic genes including UCP1 and PLIN5. These findings reveal a new biomechanical Myh9-FAK signaling arm downstream of b-adrenergic activation that differentiates thermogenic from white adipocytes.
Identifiers
PMID41409152
PMCPMC12707271
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