Evidence map›Paper›PMID 41410097›Full record

ArticleEndocrinology2025

ScRNAseq Analysis of Chicken Embryonic Pituitary Reveals Cell Heterogeneity and a Cell Type Coexpressing Gh and Pomc.

Kuan Ling Liu, Tom E Porter

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Endocrinology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Kuan Ling LiuDepartment of Animal and Avian Sciences, University of Maryland, College Park, MD 20742, USA.ORCID 0000-0002-1183-1710
Tom E PorterDepartment of Animal and Avian Sciences, University of Maryland, College Park, MD 20742, USA.ORCID 0000-0003-1168-9045

Funding

Maryland Agricultural Experiment Station MD-ANSC-222044National Institute of Food and Agriculture 2022-08009
6 · The paper itself

Abstract

Gene expression profiles and the heterogeneity among hormone-producing pituitary cells remain poorly characterized in most vertebrates, especially in chicken embryos. Using single-cell RNA sequencing, the transcriptomes of 4346 basal and 10 835 corticosterone (CORT)-treated embryonic day 11 chicken pituitary cells were sequenced. Classical endocrine cell clusters were identified, and some were shown to express previously unreported marker genes. A cluster of uncommitted cells was identified that expressed markers for multiple endocrine cell types, with ∼30% coexpressing Gh and Pomc mRNA. We named this population of cells the cortico-somatotrophs. The existence of cortico-somatotrophs were confirmed at both the mRNA and protein level. We further characterized the corticosomatotrophs by utilizing the known effect of CORT to increase somatotroph abundance. Identification of cortico-somatotrophs challenges the prevailing view that corticotrophs and somatotrophs develop from distinct cell lineages.

Indexed as

Growth HormonePituitary GlandPro-OpiomelanocortinAnimalsChick EmbryoChickensCorticosteroneCorticotrophsGene Expression Regulation, DevelopmentalRNA-SeqSequence Analysis, RNASingle-Cell AnalysisSingle-Cell Gene Expression AnalysisSomatotrophsTranscriptomeCorticosteroneGrowth HormonePro-Opiomelanocortincortico-somatotrophscorticotrophsgrowth hormonepituitaryproopiomelanocortinsomatotrophs

Identifiers

PMID41410097
PMCPMC12748939

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.