Evidence map›Paper›PMID 41413040›Full record

ArticleNature communications2025

Analysis genome of Pseudotaxus chienii reveals insights into the origin and evolution of taxane biosynthesis.

Mingshuang Wang, Ruoyun Ma, Zijin Fang, Lingxiao Zhang, Yibo Zhang, Mengyin Zheng, Enhui Bai, Wanting Lin, Yanyan Pei, Yue Zang and 8 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature communications, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Review
  3. Recent advances inFrontiers in plant science · 2026
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors.

Mingshuang WangCollege of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, Zhejiang, China.
Ruoyun MaCollege of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, Zhejiang, China.
Zijin FangCollege of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, Zhejiang, China.
Lingxiao ZhangCollege of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, Zhejiang, China.
Yibo ZhangCollege of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, Zhejiang, China.
Mengyin ZhengCollege of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, Zhejiang, China.
Enhui BaiCollege of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, Zhejiang, China.
Wanting LinCollege of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, Zhejiang, China.
Yanyan PeiCollege of Pharmacy, Hangzhou Normal University, Hangzhou, Zhejiang, China.
Yue ZangCollege of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, Zhejiang, China.
Huijie MaCollege of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, Zhejiang, China.
Shangguo FengCollege of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, Zhejiang, China.
Xiaori ZhanCollege of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, Zhejiang, China.
Chunna YuCollege of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, Zhejiang, China.
Shuling WangCollege of Pharmacy, Hangzhou Normal University, Hangzhou, Zhejiang, China.
Huizhong WangCollege of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, Zhejiang, China.
Chenliang YuNational Key Laboratory for Development and Utilization of Forest Food Resources, Zhejiang A&F University, Hangzhou, Zhejiang, China. yuchenliang@zafu.edu.cn.ORCID http://orcid.org/0000-0002-2403-4665
Chenjia ShenCollege of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, Zhejiang, China. shencj@hznu.edu.cn.ORCID http://orcid.org/0000-0002-8575-0593

Funding

National Natural Science Foundation of China (National Science Foundation of China) 32270382
6 · The paper itself

Abstract

As a sister genus to Taxus, Pseudotaxus holds significant importance for studying the origin and evolution of the taxane biosynthesis pathway. However, the reference genome of Pseudotaxus chienii is yet unavailable. Here, we report a chromosome-level genome assembly of P. chienii (15.6 Gb). We show that P. chienii only possesses a partial taxane pathway, which terminates before taxane 2α-O-benzoyl transferase (TBT), a crucial enzyme responsible for the production of 10-deacetylbaccatin III. With the emergence of the Taxus genus, the limitation posed by the lacking of functional TBT is overcome, allowing for the extension of the existing taxane biosynthesis pathway into a complete Taxol biosynthesis. The protein structure of metal ion catalysis sites in taxadiene synthase (TS) is conserved across the Pseudotaxus and Taxus genera, providing potential sites for enhancing TS activity through enzyme engineering. This comparative genomic analysis contributes to our understanding of the origin and evolution of taxane biosynthesis within the Taxaceae family.

Indexed as

Bridged-Ring CompoundsEvolution, MolecularGenome, PlantTaxoidsTaxusBiosynthetic PathwaysIsomerasesPaclitaxelPhylogenyPlant Proteins10-deacetylbaccatine IIIBridged-Ring CompoundsIsomerasesPaclitaxelPlant Proteinstaxa-4(5),11(12)-diene synthasetaxaneTaxoids

Identifiers

PMID41413040
PMCPMC12852726

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.