Evidence map›Paper›PMID 41414839›Full record

ArticleAnimal bioscience2026

MYSM1 regulates the proliferation and differentiation of bovine skeletal muscle satellite cells via BRG1-mediated activation of the AKT/mTOR/NF-κB signaling pathway.

Chujie Zhang, Yue Li, Wenwen Zhang, Tengxia Ma, Xin Li, Yiwen Guo, Linlin Zhang, Xiangbin Ding, Debao Hu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Animal bioscience, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Chujie Zhang *Key Laboratory of Animal Breeding and Healthy Livestock Farming, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, China.
Yue Li *Key Laboratory of Animal Breeding and Healthy Livestock Farming, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, China.
Wenwen ZhangKey Laboratory of Animal Breeding and Healthy Livestock Farming, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, China.
Tengxia MaKey Laboratory of Animal Breeding and Healthy Livestock Farming, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, China.
Xin LiKey Laboratory of Animal Breeding and Healthy Livestock Farming, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, China.
Yiwen GuoKey Laboratory of Animal Breeding and Healthy Livestock Farming, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, China.
Linlin ZhangKey Laboratory of Animal Breeding and Healthy Livestock Farming, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, China.
Xiangbin DingKey Laboratory of Animal Breeding and Healthy Livestock Farming, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, China. xiangbinding@tjau.edu.cn.
Debao HuKey Laboratory of Animal Breeding and Healthy Livestock Farming, College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin, China. hudebao@tjau.edu.cn.

Funding

Scientific Research Program of Tianjin Education Commission 2021KJ107
6 · The paper itself

Abstract

objectiveThis study investigates the molecular mechanisms by which the deubiquitinase MYSM1 regulates the proliferation and differentiation of bovine skeletal muscle satellite cells (BSMSCs), thereby providing new theoretical insights into the regulation of muscle growth and development in beef cattle.

methodsAn in vitro model of BSMSCs was established to investigate the role of MYSM1. The expression pattern of MYSM1 during cell proliferation and differentiation was analyzed using quantitative real-time polymerase chain reaction (qRT-PCR). MYSM1 knockdown models were generated, and the expression of proliferation markers PAX7 and Ki67, as well as differentiation markers MYHC and MYOG, were examined by qRT-PCR and Western blotting. Analysis of protein-protein interactions identified BRG1 as a potential MYSM1 interactor, and its function was subsequently evaluated. Downstream signaling activity was evaluated by examining phosphorylation changes in key components of the AKT/mTOR pathway. In addition, global histone ubiquitination H2AK119ub1 and the histone methylation markers H3K4me3 and H3K27me3 were analyzed following MYSM1 knockdown.

resultsMYSM1 expression was dynamically regulated, exhibiting significant upregulation during differentiation, reaching its highest at days 2-3 (p<0.05). Silencing of MYSM1 significantly (p<0.05) decreased the expression of PAX7, Ki67, MYHC, and MYOG. Histone modification analyses demonstrated elevated levels of H2AK119ub1 and H3K27me3, along with reduced H3K4me3 (p<0.05). Mechanistic studies showed that MYSM1 knockdown significantly reduced BRG1 expression (p<0.05), and BRG1 silencing similarly decreased proliferation and differentiation markers. Moreover, interference with either MYSM1 or BRG1 significantly attenuated the activation of the AKT/mTOR/NF-κB signaling pathway, as evidenced by decreased phosphorylation of AKT, mTOR, and p65 (p<0.05).

conclusionMYSM1 promotes the proliferation and differentiation of BSMSCs through BRG1-mediated epigenetic regulation and activation of the AKT/mTOR/NF-κB signaling cascade. These findings establish a dual-target framework that advances the understanding of muscle development in beef cattle and offers potential strategies for regenerative therapies.

Indexed as

AKT/mTOR/NF-κB Signaling PathwayBovine Skeletal Muscle Satellite CellsBRG1Epigenetic Modifications of HistonesMYSM1

Identifiers

PMID41414839
PMCPMC13243919

What Socratic holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.