ArticlePlant phenomics (Washington, D.C.)2025
Determination of optimal sampling time of grape embryo rescue based on near infrared spectroscopy combined with machine learning.
Article in Plant phenomics (Washington, D.C.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Grape embryo rescue technology is currently the primary method for breeding new seedless grape cultivars. The timing of berry sampling directly impacts the efficacy of this technique. Therefore, achieving efficient, accurate, and non-destructive determination of the optimal sampling time for seedless grape embryo rescue breeding has long been a challenge. This study collected near-infrared spectral data and data on 19 physiological indicators from 2940 grape berries of six grape cultivars at six sampling times to construct a baseline dataset. Remarkably, it was discovered for the first time that pericarp puncture hardness (PPH) is closely associated with the embryo development rate of seedless grape. Subsequently, the optimal sampling times for 'Flame Seedless', 'Ruby Seedless', and 'Jingzaojing' were determined when their PPH reached 720 ± 20 g, 990 ± 20 g and 633 ± 20 g, respectively. Then, a total of 840 models for PPH recognition were established and assessed based on their coefficient of determination (
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