Evidence mapPaperPMID 41417364Full record

Observational studyRevista da Associacao Medica Brasileira (1992)2025

Polymorphisms in the proprotein convertase subtilisin/kexin type 9 gene in clinically diagnosed patients with familial hypercholesterolemia.

Aldrina Laura da Silva Costa, José Ernesto Dos Santos, Wilson Salgado Junior, Caroline Bertoncini-Silva, Letícia Bizari, Gustavo Santos Paiva Laender Moura, Raphael Del Roio Liberatore Junior, Renato Augusto Zorzo, Vivian Marques Miguel Suen

Abstract readObservational Study
In one paragraph

Observational study in Revista da Associacao Medica Brasileira (1992), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

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4 · The record

Corrections and comments

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5 · Who and what money

Authors and funding

9 authors.

Aldrina Laura da Silva CostaUniversidade de São Paulo, Ribeirão Preto Medical School, Department of Internal Medicine - São Paulo (SP), Brazil.ORCID http://orcid.org/0000-0003-4369-6995
José Ernesto Dos SantosUniversidade de São Paulo, Ribeirão Preto Medical School, Department of Internal Medicine - São Paulo (SP), Brazil.ORCID http://orcid.org/0000-0003-3269-1582
Wilson Salgado JuniorUniversidade de São Paulo, Ribeirão Preto Medical School, Department of Internal Medicine - São Paulo (SP), Brazil.ORCID http://orcid.org/0000-0001-9796-0339
Caroline Bertoncini-SilvaUniversidade de São Paulo, Ribeirão Preto Medical School, Department of Internal Medicine - São Paulo (SP), Brazil.ORCID http://orcid.org/0000-0002-5925-5353
Letícia BizariUniversidade de São Paulo, Ribeirão Preto Medical School, Department of Internal Medicine - São Paulo (SP), Brazil.ORCID http://orcid.org/0000-0001-7187-0706
Gustavo Santos Paiva Laender MouraUniversidade de São Paulo, Ribeirão Preto Medical School, Department of Internal Medicine - São Paulo (SP), Brazil.ORCID http://orcid.org/0000-0003-1298-8557
Raphael Del Roio Liberatore JuniorUniversidade de São Paulo, Ribeirão Preto Medical School, Department of Internal Medicine - São Paulo (SP), Brazil.ORCID http://orcid.org/0000-0001-8893-2192
Renato Augusto ZorzoUniversidade de São Paulo, Ribeirão Preto Medical School, Department of Internal Medicine - São Paulo (SP), Brazil.ORCID http://orcid.org/0000-0002-7704-3936
Vivian Marques Miguel SuenUniversidade de São Paulo, Ribeirão Preto Medical School, Department of Internal Medicine - São Paulo (SP), Brazil.ORCID http://orcid.org/0000-0001-6165-5746

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveThe aim of this study was to investigate polymorphisms in the proprotein convertase subtilisin/kexin type 9 gene and compare disease severity in familial hypercholesterolemia participants with and without these polymorphisms.

methodsFifty patients were included in this observational, cross-sectional study of primary data collection between June 2014 and June 2015 at Clinics Hospital of Ribeirão Preto Medical School/University of São Paulo Inclusion criteria for the study included individuals with an low-density lipoprotein cholesterol level ≥190 mg/dL, based on Dutch make early diagnosis prevent early death criteria for familial hypercholesterolemia. Patients with kidney disease, liver failure, a triglyceride level ≥300 mg/dL, or hypothyroidism were excluded. Gene analysis was performed using the high-resolution melting method. deoxyribonucleic acid samples with detected changes were sequenced to identify polymorphisms. Lipid profile, body mass index, history of cardiovascular events, gender, and statin use were compared between participants with and without polymorphisms.

resultsOf the 50 patients, 14 carried proprotein convertase subtilisin/kexin type 9 polymorphisms. Familial hypercholesterolemia was not associated with these polymorphisms in this cohort. As gene expression was not assessed, no conclusions can be drawn regarding functional effects. Six patients (four in the polymorphism group and two in the non-polymorphism group) reported cardiovascular events. The significantly higher occurrence of these events in the polymorphism group suggests a possible association between the presence of proprotein convertase subtilisin/kexin type 9 polymorphisms and an increased cardiovascular risk.

conclusionBenign variants were identified in the proprotein convertase subtilisin/kexin type 9 gene, with no pathogenic alterations detected. Despite the significantly higher number of participants with a history of cardiovascular events in the polymorphism group, these data do not support a direct, causal association between these polymorphisms and an increased cardiovascular risk.

Indexed as

Hyperlipoproteinemia Type IIPolymorphism, GeneticProprotein Convertase 9AdultCross-Sectional StudiesFemaleHumansMaleMiddle AgedSeverity of Illness IndexPCSK9 protein, humanProprotein Convertase 9

Identifiers

PMID41417364
PMCPMC12711128

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.