Evidence map›Paper›PMID 41427883›Full record

ArticleSleep2026

Region-specific pericyte Ca2+ transient dynamics and capillary hemodynamics during the sleep-wake cycle.

Yan Wu, Pengfei Li, Liu Liu, Wenyu Gou, Hongkuan Fan, Hongjun Wang, Meng Liu

Abstract read
In one paragraph

Article in Sleep, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

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0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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5 · Who and what money

Authors and funding

7 authors.

Yan WuDepartment of Psychiatry and Behavioral Sciences, Medical University of South Carolina, Charleston, SC 29425, United States.
Pengfei LiDepartment of Pathology, Medical University of South Carolina, Charleston, SC 29425, United States.
Liu LiuDepartment of Pathology, Medical University of South Carolina, Charleston, SC 29425, United States.
Wenyu GouDepartment of Surgery, Medical University of South Carolina, Charleston, SC 29425, United States.
Hongkuan FanDepartment of Pathology, Medical University of South Carolina, Charleston, SC 29425, United States.ORCID 0000-0002-9441-9405
Hongjun WangDepartment of Surgery, Medical University of South Carolina, Charleston, SC 29425, United States.
Meng LiuDepartment of Psychiatry and Behavioral Sciences, Medical University of South Carolina, Charleston, SC 29425, United States.

Funding

The Role of Pericytes in Brain Hypoperfusion in Alzheimer's Disease DevelopmentR01AG081807 · NIA · MEDICAL UNIVERSITY OF SOUTH CAROLINA · PI Hongkuan Fan · 2023 to 2026
$2.5M
Sleep, Pericytes, and Alzheimer's DiseaseRF1AG077570 · NIA · MEDICAL UNIVERSITY OF SOUTH CAROLINA · PI LIU, MENG · 2022 to 2022
$2.0M
Sleep, Pericytes, and Alzheimer's DiseaseR01AG077570 · NIA · MEDICAL UNIVERSITY OF SOUTH CAROLINA · PI Meng Liu · 2025 to 2026
$1.2M
Cellular Mechanism underlying emotional problems of Alzheimer's diseaseR21AG067445 · NIA · MEDICAL UNIVERSITY OF SOUTH CAROLINA · PI LIU, MENG · 2020 to 2021
$411k
NIA NIH HHS 1RF1AG077570NIA NIH HHS R01AG076777NIA NIH HHS R01 AG077570NIA NIH HHS R01 AG081807NIA NIH HHS R21 AG067445NIA NIH HHS R21AG067445NIA NIH HHS RF1 AG077570
6 · The paper itself

Abstract

STUDY

objectivesBrain pericytes play a crucial role in neurovascular coupling, regulating cerebral blood flow in response to changes in local neuronal activity. However, their activities during sleep remain less understood. Our objective is to investigate the dynamic changes in pericyte activity and related hemodynamic changes during the sleep-wake cycle.

methodsUsing an in vivo calcium imaging tool, we recorded cytosolic calcium transient dynamics from brain pericytes across natural sleep-wake cycles in the prefrontal cortex and lateral hypothalamus of mice expressing the calcium sensor GCaMP6f specifically in pericytes. Using an in vivo vascular imaging tool, capillary diameter and red blood cell velocity dynamic changes in prefrontal cortex and lateral hypothalamus were recorded and calculated across natural sleep-wake cycles.

resultsOur results show that prefrontal cortex pericytes exhibit low basal calcium transients during non-rapid eye movement sleep and rapid eye movement sleep, while showing synchronous calcium peaks following transitions from sleep to waking. In contrast, lateral hypothalamus pericytes display diverse calcium transient patterns across the sleep-wake cycle, with 81.3%, 15.6%, and 56.3% presenting calcium peaks during non-rapid eye movement sleep, rapid eye movement sleep, and waking, respectively. Hemodynamic recordings in prefrontal cortex demonstrated greater fluctuations in red blood cell velocity during waking compared to non-rapid eye movement sleep, likely associated with periodic pericyte activations during waking. In lateral hypothalamus, average capillary diameter and red blood cell velocity significantly increased during waking compared to non-rapid eye movement sleep, aligning with the predominantly non-rapid eye movement sleep-ON pattern of pericyte calcium transients and frequent capillary stalling observed during non-rapid eye movement sleep.

conclusionsOur findings identified the region-specific pericyte activity patterns that may contribute to local cerebral blood flow dynamics during natural sleep-wake cycles, emphasizing their importance in maintaining neurovascular function in different vigilant states.

Indexed as

CalciumCapillariesCerebrovascular CirculationHemodynamicsPericytesSleepWakefulnessAnimalsCalcium SignalingHypothalamusMaleMiceMice, Inbred C57BLPrefrontal CortexCalciumcalcium imagingcapillaryhemodynamicspericytessleep

Identifiers

PMID41427883
PMCPMC12928733

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.