Evidence map›Paper›PMID 41430435›Full record

ArticleCommunications biology2025

Longitudinal human transcriptomic and spatial gene profiling at the incisional edge during long surgical procedures.

Matthew R Sapio, Evelyn Li, Anthony F Domenichiello, Taichi Goto, Dragan Maric, Allison P Manalo, Tracy S Williams, Saber Tadros, Jonathan M Hernandez, David S Schrump and 4 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Communications biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Matthew R SapioDepartment of Perioperative Medicine, National Institutes of Health, Clinical Center, Bethesda, MD, USA.ORCID http://orcid.org/0000-0002-8855-5419
Evelyn LiDepartment of Perioperative Medicine, National Institutes of Health, Clinical Center, Bethesda, MD, USA.
Anthony F DomenichielloLipid Peroxidation Unit, Laboratory of Clinical Investigation, National Institute on Aging, National Institutes of Health, Baltimore, MD, USA.ORCID http://orcid.org/0000-0003-4808-8403
Taichi GotoSymptoms Biology Unit, National Institute of Nursing Research, National Institutes of Health, Bethesda, MD, USA.ORCID http://orcid.org/0000-0001-7140-8175
Dragan MaricFlow and Imaging Cytometry Core Facility, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD, USA.ORCID http://orcid.org/0000-0003-2912-7921
Allison P ManaloDepartment of Perioperative Medicine, National Institutes of Health, Clinical Center, Bethesda, MD, USA.ORCID http://orcid.org/0000-0003-3616-5416
Tracy S WilliamsDepartment of Perioperative Medicine, National Institutes of Health, Clinical Center, Bethesda, MD, USA.
Saber TadrosDepartment of Pathology, Microbiology and Immunology, University of Nebraska Medical Center, Omaha, NE, USA.
Jonathan M HernandezSurgical Oncology Program, Center for Cancer Research, National Cancer Institute, Bethesda, MD, USA.
David S SchrumpThoracic Surgery Branch, Center for Cancer Research, National Cancer Institute, Bethesda, MD, USA.
Jeremy L DavisSurgical Oncology Program, Center for Cancer Research, National Cancer Institute, Bethesda, MD, USA.ORCID http://orcid.org/0000-0002-6334-6936
Andrew M BlakelySurgical Oncology Program, Center for Cancer Research, National Cancer Institute, Bethesda, MD, USA.ORCID http://orcid.org/0000-0002-5013-3851
Michael J IadarolaDepartment of Perioperative Medicine, National Institutes of Health, Clinical Center, Bethesda, MD, USA.ORCID http://orcid.org/0000-0001-7188-9810
Andrew J MannesDepartment of Perioperative Medicine, National Institutes of Health, Clinical Center, Bethesda, MD, USA. amannes@cc.nih.gov.ORCID http://orcid.org/0000-0001-5834-5667

Funding

U.S. Department of Health & Human Services | NIH | NIH Clinical Center (Clinical Center) ZIACL090034-09, ZIACL090035-08, ZIACL0033-09
6 · The paper itself

Abstract

Post-surgical pain remains a widespread problem reducing quality of life. The present study investigates the initial molecular changes underlying nociceptive sensitization through longitudinal, temporal sampling at the surgical wound edge. Using RNA-Seq and multiplex fluorescence in situ hybridization, we examined the most significant genes induced by tissue injury including those coding for the secreted factors interleukin 6, oncostatin M and leukemia inhibitory factor, and localized these induction events to several cutaneous structures including the epidermis, vascular endothelia, hair follicles, and sweat glands. Our data also demonstrate the receptors for these key secreted factors are expressed by dorsal root ganglion neurons, indicating long-range signaling from damaged skin to spinal cord, thereby leading to pain. This study provides a novel understanding of tissue structures and the molecular interactome activated following tissue injury by elucidating the inflammatory and tissue repair transcriptional milieu induced by surgery in human skin excision biopsies.

Indexed as

Surgical WoundTranscriptomeAdultFemaleGene Expression ProfilingHumansLongitudinal StudiesMaleMiddle AgedSkinWound Healing

Identifiers

PMID41430435
PMCPMC12936088

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.