Evidence map›Paper›PMID 41432570›Full record

ArticlePlant biotechnology journal2026

Cas9-Embedding Hyperactive TadA8e Confers Efficient and Highly Specific A-To-G Base Editing in Rice.

Jianjian Hu, Xue Li, Yuhong Gao, Yifan Guo, Yini Liu, Chen Wang, Gencheng Xu, Chaoyue Du, Shijia Liu, Zhigang Zhao and 5 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plant biotechnology journal, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Jianjian HuState Key Laboratory of Crop Genetics and Germplasm Enhancement and Utilization, Sanya Institute of Nanjing Agricultural University, Jiangsu Engineering Research Center for Plant Genome Editing, National Observation and Research Station of Rice Germplasm Resources, Nanjing Agricultural University, Nanjing, China.
Xue LiState Key Laboratory of Crop Genetics and Germplasm Enhancement and Utilization, Bioinformatics Center, Academy for Advanced Interdisciplinary Studies, Nanjing Agricultural University, Nanjing, China.
Yuhong GaoState Key Laboratory of Crop Genetics and Germplasm Enhancement and Utilization, Sanya Institute of Nanjing Agricultural University, Jiangsu Engineering Research Center for Plant Genome Editing, National Observation and Research Station of Rice Germplasm Resources, Nanjing Agricultural University, Nanjing, China.
Yifan GuoState Key Laboratory of Crop Genetics and Germplasm Enhancement and Utilization, Sanya Institute of Nanjing Agricultural University, Jiangsu Engineering Research Center for Plant Genome Editing, National Observation and Research Station of Rice Germplasm Resources, Nanjing Agricultural University, Nanjing, China.
Yini LiuState Key Laboratory of Crop Genetics and Germplasm Enhancement and Utilization, Sanya Institute of Nanjing Agricultural University, Jiangsu Engineering Research Center for Plant Genome Editing, National Observation and Research Station of Rice Germplasm Resources, Nanjing Agricultural University, Nanjing, China.
Chen WangState Key Laboratory of Crop Genetics and Germplasm Enhancement and Utilization, Sanya Institute of Nanjing Agricultural University, Jiangsu Engineering Research Center for Plant Genome Editing, National Observation and Research Station of Rice Germplasm Resources, Nanjing Agricultural University, Nanjing, China.
Gencheng XuState Key Laboratory of Crop Genetics and Germplasm Enhancement and Utilization, Sanya Institute of Nanjing Agricultural University, Jiangsu Engineering Research Center for Plant Genome Editing, National Observation and Research Station of Rice Germplasm Resources, Nanjing Agricultural University, Nanjing, China.
Chaoyue DuState Key Laboratory of Crop Genetics and Germplasm Enhancement and Utilization, Sanya Institute of Nanjing Agricultural University, Jiangsu Engineering Research Center for Plant Genome Editing, National Observation and Research Station of Rice Germplasm Resources, Nanjing Agricultural University, Nanjing, China.
Shijia LiuState Key Laboratory of Crop Genetics and Germplasm Enhancement and Utilization, Sanya Institute of Nanjing Agricultural University, Jiangsu Engineering Research Center for Plant Genome Editing, National Observation and Research Station of Rice Germplasm Resources, Nanjing Agricultural University, Nanjing, China.
Zhigang ZhaoState Key Laboratory of Crop Genetics and Germplasm Enhancement and Utilization, Sanya Institute of Nanjing Agricultural University, Jiangsu Engineering Research Center for Plant Genome Editing, National Observation and Research Station of Rice Germplasm Resources, Nanjing Agricultural University, Nanjing, China.
Yihua WangState Key Laboratory of Crop Genetics and Germplasm Enhancement and Utilization, Sanya Institute of Nanjing Agricultural University, Jiangsu Engineering Research Center for Plant Genome Editing, National Observation and Research Station of Rice Germplasm Resources, Nanjing Agricultural University, Nanjing, China.ORCID https://orcid.org/0000-0003-1945-1277
Yufeng WuState Key Laboratory of Crop Genetics and Germplasm Enhancement and Utilization, Bioinformatics Center, Academy for Advanced Interdisciplinary Studies, Nanjing Agricultural University, Nanjing, China.
Xiaoou DongState Key Laboratory of Crop Genetics and Germplasm Enhancement and Utilization, Sanya Institute of Nanjing Agricultural University, Jiangsu Engineering Research Center for Plant Genome Editing, National Observation and Research Station of Rice Germplasm Resources, Nanjing Agricultural University, Nanjing, China.
Chao LiState Key Laboratory of Crop Genetics and Germplasm Enhancement and Utilization, Sanya Institute of Nanjing Agricultural University, Jiangsu Engineering Research Center for Plant Genome Editing, National Observation and Research Station of Rice Germplasm Resources, Nanjing Agricultural University, Nanjing, China.
Jianmin WanState Key Laboratory of Crop Genetics and Germplasm Enhancement and Utilization, Sanya Institute of Nanjing Agricultural University, Jiangsu Engineering Research Center for Plant Genome Editing, National Observation and Research Station of Rice Germplasm Resources, Nanjing Agricultural University, Nanjing, China.

Funding

Ministry of Education of the People's Republic of Chinathe Bingtuan Key Science and Technology Program of Xinjiang Province 2023AB006-02the Biological Breeding-Major Projects 2023ZD04074the Fundamental Research Funds for the Central Universities KYT2024005the Guidance Foundation of the Sanya Institute of Nanjing Agricultural University NAUSY-ZZ03the Jiangsu Province Natural Science Foundation BK20230038the Nanjing U35 programthe National Key Research and Development Program 2023YFD1202900the National Natural Science Foundation of China 31872806the Zhongshan Biological Breeding Laboratory ZSBBL-KY2023-04
6 · The paper itself

Abstract

Adenine base editors (ABEs) produce precise A-to-G conversion in the genomic target sites without causing double-strand breaks. However, the hyperactive adenosine deaminase TadA8e raises safety concerns on genome-wide off-target edits. We engineered 11 chimeric proteins for ABEs (CP-ABEs) by embedding hyperactive TadA8e within Cas9 nickase to minimise the sgRNA-independent off-target effects. Four CP-ABEs exhibited robust on-target activity with minimal sgRNA-independent off-target edits. Then we developed four chimeric high-fidelity ABEs (CH-ABEs) to minimise both sgRNA-dependent and sgRNA-independent off-target effects by employing high-fidelity Cas9 variants. The CH-ABEs achieved reductions of up to 7.0-fold and 79.4-fold in the respective off-target edits, while generating 22.0%-72.4% homozygous and biallelic rice mutants. Whole-genome and whole-transcriptome sequencing (WGS/WTS) confirmed the specificity of CH-ABEs. Incorporating Sniper2L into CH-ABEs further enhanced both specificity and on-target activity. Two PAM-less SpRY variants (SpRY-K2, SpRY-KK) expanded the targeting scope of CP-ABEs and boosted activity by 80.0%. Furthermore, we demonstrated that CP-ABE8e-RY

Indexed as

Adenosine DeaminaseCRISPR-Associated Protein 9Gene EditingOryzaCRISPR-Cas SystemsGenome, PlantPlant ProteinsPlants, Genetically ModifiedRNA, Guide, CRISPR-Cas SystemsAdenosine DeaminaseCRISPR-Associated Protein 9Plant ProteinsRNA, Guide, CRISPR-Cas Systemsadenine base editorCH‐ABEhigh‐fidelitySpRYTadA8e

Identifiers

PMID41432570
PMCPMC13140646

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.