Evidence map›Paper›PMID 41436618›Full record

ArticleScientific reports2025

Development and application of SNP markers to discriminate Korean Perilla (Perilla frutescens) varieties using genomic sequence variations.

Jung-In Kim, Myoung Hee Lee, Sang Woo Kim, Eunsoo Lee, Sungup Kim, Jeongeun Lee, Heungsu Lee, Ki Young Kim, Eunyoung Oh, Min Young Kim and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Jung-In KimUpland Crop Breeding Division, National Institute of Crop and Food Science, Rural Development Administration, Miryang, 50424, Republic of Korea.
Myoung Hee LeeUpland Crop Breeding Division, National Institute of Crop and Food Science, Rural Development Administration, Miryang, 50424, Republic of Korea.
Sang Woo KimUpland Crop Breeding Division, National Institute of Crop and Food Science, Rural Development Administration, Miryang, 50424, Republic of Korea.
Eunsoo LeeUpland Crop Breeding Division, National Institute of Crop and Food Science, Rural Development Administration, Miryang, 50424, Republic of Korea.
Sungup KimUpland Crop Breeding Division, National Institute of Crop and Food Science, Rural Development Administration, Miryang, 50424, Republic of Korea.
Jeongeun LeeUpland Crop Breeding Division, National Institute of Crop and Food Science, Rural Development Administration, Miryang, 50424, Republic of Korea.
Heungsu LeeUpland Crop Breeding Division, National Institute of Crop and Food Science, Rural Development Administration, Miryang, 50424, Republic of Korea.
Ki Young KimUpland Crop Breeding Division, National Institute of Crop and Food Science, Rural Development Administration, Miryang, 50424, Republic of Korea.
Eunyoung OhValue Crop Research Institute, National Institute of Crop and Food Science, Rural Development Administration, Muan, 58545, Republic of Korea.
Min Young KimFood Tech Resources Research Division, National Institute of Crop and Food Science, Rural Development Administration, Wanju, 55365, Republic of Korea.
Kwang-Soo ChoHighland Agriculture Research Institute, National Institute of Crop and Food Science, Rural Development Administration, Pyeongchang, 25342, Republic of Korea. kscholove@korea.kr.
Tae-Hwan JunDepartment of Plant Bioscience, Pusan National University, Miryang, 50463, Republic of Korea. thjun76@pusan.ac.kr.

Funding

Rural Development Administration PJ016076012023
6 · The paper itself

Abstract

Perilla [Perilla frutescens (L.) Britton] is an annual herbaceous species of the Lamiaceae family native to Northeast Asia, cultivated for both seed oil production and as a leafy vegetable. We developed and validated Kompetitive Allele-Specific PCR (KASP) markers for accurate identification of Korean Perilla cultivars. Whole-genome resequencing of 16 representative cultivars yielded 9686,199 SNPs, with 6183 high-confidence SNPs identified after stringent filtering. From these, 237 KASP markers were designed, and 150 polymorphic markers were validated across 48 cultivars. Principal coordinate analysis (PCoA) and phylogenetic analyses mostly distinguished seed-type from leaf-type Perilla. Minimal KASP marker sets (five for seed Perilla, six for leaf Perilla) were established, sufficient to distinguish widely cultivated Korean cultivars. These markers, encoded by a binary barcode system, enabled rapid and precise cultivar identification. Application tests demonstrated their utility for evaluating seed purity by quantifying contamination. This work provides substantial genomic resources for cultivar authentication, genetic purity assessment, and molecular breeding. The new KASP system offers a cost-effective, high-throughput, and reliable approach for managing and enhancing Perilla genetic resources, ultimately advancing breeding programs and improving seed industry processes.

Indexed as

Genome, PlantPerilla frutescensPolymorphism, Single NucleotideAllelesGenetic MarkersGenomicsPhylogenyRepublic of KoreaSeedsGenetic MarkersCultivar identificationGenetic diversityKASP markersPerilla frutescensSeed purity testing

Identifiers

PMID41436618
PMCPMC12830944

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.