Evidence map›Paper›PMID 41436981›Full record

ArticleBMC cancer2025

Role of histone acetylation in the regulation of CD36 and ANGPTL1 in esophageal squamous cell carcinoma.

Aadil Manzoor Baba, Ritis Kumar Shyanti, Mohammad Amin Hajam, Najma Nissa, Mansha Muzaffar, Tanzeela Bashir, Sauliheen Fida, Mudabir Abdullah, Rana P Singh, Nazir Ahmad Dar

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Article in BMC cancer, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

10 authors.

Aadil Manzoor BabaDepartment of Biochemistry, University of Kashmir, Srinagar, India.
Ritis Kumar ShyantiSchool of Life Science, Jawaharlal Nehru University, New Delhi, India.
Mohammad Amin HajamDepartment of Biochemistry, University of Kashmir, Srinagar, India.
Najma NissaDepartment of Biochemistry, University of Kashmir, Srinagar, India.
Mansha MuzaffarDepartment of Biochemistry, University of Kashmir, Srinagar, India.
Tanzeela BashirDepartment of Biochemistry, University of Kashmir, Srinagar, India.
Sauliheen FidaDepartment of Biochemistry, University of Kashmir, Srinagar, India.
Mudabir AbdullahDepartment of Biochemistry, University of Kashmir, Srinagar, India.
Rana P SinghSchool of Life Science, Jawaharlal Nehru University, New Delhi, India.
Nazir Ahmad DarDepartment of Biochemistry, University of Kashmir, Srinagar, India. nazirramzan@uok.edu.in.

Funding

DST-FIST Grant No SR/FST/LS-1/2017/3).(DST-SERB), Government of India. File no. CRG/2018/000202
6 · The paper itself

Abstract

backgroundGiven the limited efficacy of available anti-angiogenic therapies in esophageal squamous cell carcinoma (ESCC), there is a need to explore new strategies to target this crucial tumour-promoting process. Emerging evidence suggests that epigenetic dysregulation, including histone deacetylation, may contribute to aberrant pro-angiogenic signalling. Therefore, we investigated whether histone deacetylase (HDAC) 3 and HDAC8-mediated hypoacetylation contribute to the silencing of anti-angiogenic genes angiopoietin-like 1 (ANGPTL1) and cluster of differentiation (CD36), thereby promoting angiogenesis in ESCC.

methodsESCC cell lines and clinical tissues were analysed for the expression of CD36, ANGPTL1, vascular endothelial growth factor (VEGF), HDAC3 and HDAC8 and for histone acetylation markers histone H3 lysine 9 acetylation (H3K9ac) and histone H4 lysine 16 acetylation (H4K16ac) using qRT-PCR and immunoblotting. Functional angiogenesis was assessed through in vitro human umbilical vein endothelial cell (HUVEC) tube formation, ex vivo rat aortic ring assays, and endothelial migration (wound healing/Trans well) following interventions with suberoylanilide hydroxamic acid (SAHA) treatment, HDAC3 siRNA knockdown, or CD36 overexpression.

resultsIn ESCC tissues and cell lines, expression of CD36 and ANGPTL1 was downregulated, while HDAC3 and HDAC8 were upregulated, correlating with reduced levels of H3K9ac and H4K16ac. The HDAC inhibitor SAHA partially restored histone acetylation, significantly upregulated CD36 and ANGPTL1, suppressed VEGF, and reduced angiogenesis in both in vitro and ex vivo models. Furthermore, knockdown of HDAC3 produced a significant anti-angiogenic effect, comparable to the effect of CD36 overexpression. In both cases, conditioned media from the modified cells impaired endothelial cell migration and tube formation.

Indexed as

Angiopoietin-like ProteinsCarcinoma, Squamous CellCD36 AntigensEsophageal NeoplasmsEsophageal Squamous Cell CarcinomaHistonesAcetylationAngiopoietin-Like Protein 1AnimalsCell Line, TumorCell MovementGene Expression Regulation, NeoplasticHistone Deacetylase 3Histone DeacetylasesHumansHuman Umbilical Vein Endothelial CellsAngiopoietin-Like Protein 1Angiopoietin-like ProteinsANGPTL1 protein, humanCD36 AntigensCD36 protein, humanHDAC8 protein, humanHistone Deacetylase 3Histone DeacetylasesHistonesRepressor ProteinsVascular Endothelial Growth Factor AAngiogenesisH3K9acH4K16acHDACsTube formation and SAHA

Identifiers

PMID41436981
PMCPMC12837907

What Socratic holds

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LicenceCC BY-NC-ND
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.