Evidence map›Paper›PMID 41437219›Full record

ArticleBMC plant biology2025

Ectopic expression of maize gene ZmDUF1645 in rice affects rice grain traits and cold tolerance.

Yulin Chen, Xingzhuo Sun, Peng Zhang, Yanting Li, Tongyue Ouyang, Lihua Li, Jianqing Zhu, Xiaomei Jia, Xiaoying Ye, Jun Zhu and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC plant biology, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Plants (Basel, Switzerland) · 2026
    Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Yulin ChenState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute of Sichuan Agricultural University, Chengdu, 611130, China.
Xingzhuo SunState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute of Sichuan Agricultural University, Chengdu, 611130, China.
Peng ZhangState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute of Sichuan Agricultural University, Chengdu, 611130, China.
Yanting LiState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute of Sichuan Agricultural University, Chengdu, 611130, China.
Tongyue OuyangState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute of Sichuan Agricultural University, Chengdu, 611130, China.
Lihua LiState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute of Sichuan Agricultural University, Chengdu, 611130, China.
Jianqing ZhuDemonstration Base for International Science & Technology Cooperation of Sichuan Province, Sichuan Agricultural University, 211, Huimin Road, Chengdu, 611130, China.
Xiaomei JiaDemonstration Base for International Science & Technology Cooperation of Sichuan Province, Sichuan Agricultural University, 211, Huimin Road, Chengdu, 611130, China.
Xiaoying YeDemonstration Base for International Science & Technology Cooperation of Sichuan Province, Sichuan Agricultural University, 211, Huimin Road, Chengdu, 611130, China.
Jun ZhuState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute of Sichuan Agricultural University, Chengdu, 611130, China.
Rongjun ChenState Key Laboratory of Crop Gene Exploration and Utilization in Southwest China, Rice Research Institute of Sichuan Agricultural University, Chengdu, 611130, China. 13782@sicau.edu.cn.

Funding

Natural Science Foundation of Sichuan Province 2024NSFSC0342
6 · The paper itself

Abstract

The DUF protein family is a class of functional proteins containing conserved structural domains that are widely involved in biological processes such as plant seed development, yield formation and response to adversity. Previous research has demonstrated that the heterologous expression of ZmDUF1645 from maize (Zea mays) in rice (Oryza sativa L.) markedly enhances grain yield and influences drought tolerance. However, its effects under other stress conditions remain poorly understood. This study aims to elucidate the role of ZmDUF1645 in rice grain traits and cold tolerance. To this end, the maize gene ZmDUF1645 was expressed in rice, resulting in transgenic plants that exhibited significantly increased yield but reduced grain quality. Further analysis revealed that ZmDUF1645, with functional conservation to its rice homolog OsSGL, exerts pleiotropic effects when ectopically overexpressed in rice. It enhances yield by upregulating GIF1 to promote panicle development, increase grains per panicle, and elevate thousand-grain weight. However, it impairs grain quality (reduced amylose, abnormal starch granules, higher chalkiness) via repressing GBSS1/AGPS2, and compromises cold tolerance by disrupting redox homeostasis, inhibiting superoxide dismutase - SOD; peroxidase - POD; proline - Pro, and downregulating genes related to starch synthesis/energy metabolism. These findings highlight the pleiotropic effects of ZmDUF1645 on rice yield, quality, and stress resistance, offering valuable insights for future crop genetic improvement.

Indexed as

Ectopic Gene ExpressionEdible GrainOryzaPlant ProteinsZea maysCold TemperatureGene Expression Regulation, PlantGenes, PlantPlants, Genetically ModifiedPlant ProteinsCold toleranceGrain qualityGrain traitHeterologous expressionRice

Identifiers

PMID41437219
PMCPMC12837619

What Socratic holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.