Evidence mapPaperPMID 41438809Full record

ArticleContemporary oncology (Poznan, Poland)2025

The activity of pyrazoline B compound in inhibiting proliferation of breast cancer cells with human epidermal growth factor receptor 2 overexpression.

Denny Satria, Syukur Berkat Waruwu, Eti Nurwening Sholikhah, Mustofa Mustofa, Pamungkas Bagus Satriyo, Tutik Dwi Wahyuningsih, Hesti L Wiraswati, Muhammad Hasan Bashari, Ema Damayanti

Abstract read
In one paragraph

Article in Contemporary oncology (Poznan, Poland), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. In silico approach andContemporary oncology (Poznan, Poland) · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Denny SatriaDepartment of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Sumatera Utara, Medan, Indonesia.
Syukur Berkat WaruwuPharmacist Professional Education, Faculty of Pharmacy and Health Sciences, Universitas Sari Mutiara Indonesia, Medan, Indonesia.
Eti Nurwening SholikhahDepartment of Pharmacology, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia.
Mustofa MustofaDepartment of Pharmacology, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia.
Pamungkas Bagus SatriyoDepartment of Pharmacology, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia.
Tutik Dwi WahyuningsihDepartment of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada, Yogyakarta, Indonesia.
Hesti L WiraswatiParasitology Division, Department of Biomedical Science, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia.
Muhammad Hasan BashariPharmacology Division, Department of Biomedical Science, Faculty of Medicine, Universitas Padjadjaran, Bandung, Indonesia.
Ema DamayantiResearch Center for Food Technology and Processing, National Research and Innovation Agency, Gunungkidul, Indonesia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Introduction: This study aimed to evaluate the cytotoxicity and elucidate the mechanism of action of the chemical compound pyrazoline B in inhibiting the proliferation of MCF 7/HER-2 breast cancer cells. Material and methods: Pyrazoline B was synthesized at the Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Gadjah Mada. Cytotoxic activity against MCF 7/HER-2 cells was assessed using the MTT assay. Flow cytometry was used to analyse cell cycle arrest (G2/M phase), induction of apoptosis and necrosis, and the expression of PI3K, mTOR, and reactive oxygen species (ROS). Results: Pyrazoline B exhibited cytotoxicity with an IC Conclusions: Pyrazoline B inhibited cell cycle progression, induced apoptosis and necrosis, downregulated PI3K/mTOR signalling, and elevated ROS levels in MCF 7/HER-2 cells compared with the untreated control cells. These findings suggest that pyrazoline B is a promising candidate for development as a novel anticancer agent.

Indexed as

apoptosisbreast cancercytotoxicitygrowth inhibitionpyrazoline B

Identifiers

PMID41438809
PMCPMC12721237

What Socratic holds

Textmetadata
LicenceCC BY-NC-SA
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.