Evidence map›Paper›PMID 41444836›Full record

ArticleBritish journal of cancer2026

Pan-cancer analysis of pyrimidine metabolism reveals signaling pathways connections with chemoresistance role.

Vignesh Ramesh, Mert Demirdizen, Luisa Pinna, Thomas Koed Doktor, Federica Benso, Mohammad Aarif Siddiqui, Paolo Ceppi

Abstract read
In one paragraph

Article in British journal of cancer, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Vignesh Ramesh *Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense, Denmark.
Mert Demirdizen *Department of Biochemistry and Molecular Biology, University of Southern Denmark, Odense, Denmark.
Luisa PinnaDepartment of Biochemistry and Molecular Biology, University of Southern Denmark, Odense, Denmark.
Thomas Koed DoktorDepartment of Biochemistry and Molecular Biology, University of Southern Denmark, Odense, Denmark.
Federica BensoDepartment of Oncology, University of Turin, San Luigi Gonzaga Hospital, Orbassano, Italy.
Mohammad Aarif SiddiquiDepartment of Biochemistry and Molecular Biology, University of Southern Denmark, Odense, Denmark.ORCID http://orcid.org/0000-0003-4308-3398
Paolo CeppiDepartment of Biochemistry and Molecular Biology, University of Southern Denmark, Odense, Denmark. pceppi@bmb.sdu.dk.ORCID http://orcid.org/0000-0002-3632-8476

Funding

Deutsche Forschungsgemeinschaft (German Research Foundation) CE 281/6-1Kræftens Bekæmpelse (Danish Cancer Society) A18859Lundbeckfonden (Lundbeck Foundation) R380-2021-1264Novo Nordisk Fonden (Novo Nordisk Foundation) 0066909
6 · The paper itself

Abstract

backgroundDeregulated pyrimidine metabolism (PyMet) contributes to various tumorigenic features of cancer, including chemoresistance and epithelial-to-mesenchymal transition. However, cancer often encompasses complex signalling and metabolic pathway cascades for its progression and understanding of these molecular regulatory processes in PyMet is quite limited.

methodsA comprehensive pan-cancer analysis of around 10,000 gene expression profiles of 32 cancer types was employed using a pathway-based approach utilising gene-sets of signalling and metabolic pathways. The findings were validated using in vitro inhibitor treatments, genetic perturbations and mouse-derived lung tumour organoids.

resultsPan-cancer analysis identified several top connections with PyMet, including TERT, MTOR, DAX1, HOXA1, TP53 and TNC, implying an interdependency of regulations, which in turn was linked to the chemoresistance mechanisms. Further, these PyMet-signalling interactions were validated in vitro by inhibiting thymidylate synthase (TS) activity using knockdown approach and by brequinar (BRQ), a DHODH inhibitor. Strikingly, the BRQ treatment profile showed a strong inverse association pattern with doxorubicin chemoresistance in multiple cancer types. Indeed, BRQ synergistically sensitises cells to doxorubicin in both lung cancer cell lines and mouse-derived Kras

conclusionsThe study highlights the PyMet-pathway interactions and its role in chemoresistance, providing a strategy for targeting PyMet in cancer.

Indexed as

Drug Resistance, NeoplasmNeoplasmsPyrimidinesAnimalsCell Line, TumorDoxorubicinGene Expression Regulation, NeoplasticHumansLung NeoplasmsMiceSignal TransductionDoxorubicinpyrimidinePyrimidines

Identifiers

PMID41444836
PMCPMC12905165

What Socratic holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.