Evidence map›Paper›PMID 41454809›Full record

SynthesisThe Indian journal of medical research2025

Unveiling the phenotypic impact of cryopreservation on adipose derived stem cells: A systematic review.

Sadia Farhana, Mohd Zulkifli Salleh, Shazana H Shamsuddin, Wan Azman Wan Sulaiman, Nur Azida Mohd Nasir

Abstract readSystematic Review
PubMed Full text at PMC Publisher
In one paragraph

Synthesis in The Indian journal of medical research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Sadia FarhanaDepartment of Plastic and Reconstructive Sciences Unit, School of Medical Sciences, Health Campus, Universiti Sains Malaysia, Malaysia.
Mohd Zulkifli SallehDepartment of Medical Microbiology & Parasitology, School of Medical Sciences, Health Campus, Universiti Sains Malaysia, Malaysia.
Shazana H ShamsuddinDepartment of Pathology, School of Medical Sciences, Health Campus, Universiti Sains Malaysia, Malaysia.
Wan Azman Wan SulaimanDepartment of Plastic and Reconstructive Sciences Unit, School of Medical Sciences, Health Campus, Universiti Sains Malaysia, Malaysia.
Nur Azida Mohd NasirDepartment of Plastic and Reconstructive Sciences Unit, School of Medical Sciences, Health Campus, Universiti Sains Malaysia, Malaysia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Background & objectivesThe impact of cell passaging and cryopreservation on the phenotypic and functional attributes of adipose-derived stem cells (ADSCs) must be understood to improve their clinical value. This systematic review investigates the phenotypic characteristics of ADSCs derived from fresh and cryopreserved adipose tissue, with a focus on how these cells change across passages. MethodsA thorough search of databases was conducted as per the PRISMA guideline to find publications that were aligned with the inclusion criteria and were published between January 2013 and January 2023. ResultsIn both cryopreserved and fresh stromal vascular fraction (SVF) cells, CD90, CD73, and CD105 consistently exhibited strong expression (90%) across passages in 50 screened studies. In fresh tissue, CD29 was upregulated in subsequent passages (up to 95%) but downregulated at passage 2 (2.3%). Variable CD29 was seen in cryopreserved groups (47% at P1, 90% at P4). While CD34 and CD45 were lower in cryopreserved ADSCs (less than 5%), they were higher in fresh tissue (41%), suggesting less haematopoietic contamination. Interpretation & conclusionsPassaging and cryopreservation protocols can help maintain the therapeutic potential of ADSCs, providing a reliable source of functional stem cells for regenerative utilization.

Indexed as

Adipose TissueCryopreservationStem Cells5'-NucleotidaseCell DifferentiationEndoglinGPI-Linked ProteinsHumansMesenchymal Stem CellsPhenotypeStromal Vascular FractionThy-1 Antigens5'-NucleotidaseEndoglinGPI-Linked ProteinsNT5E protein, humanThy-1 AntigensAdipose-derived stem cellscluster of differentiationcryopreservationimmunophenotypessystematic review

Identifiers

PMID41454809
PMCPMC12744540

What Socratic holds

Textmetadata
LicenceCC BY-NC-SA
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.