Evidence map›Paper›PMID 41454948›Full record

ArticleMikrochimica acta2025

Advancement in continuous lactate determination in untreated human serum: multipolymer-based amperometric biosensor coupled with a low-cost 3D-printed microfluidic cell.

Álvaro Jesús Sainz-Calvo, Alfonso Sierra-Padilla, Dolores Bellido-Milla, Lorena Blanco-Díaz, Juan Jesús Fernández-Alba, Carmen González-Macías, Juan José García-Guzmán, José María Palacios-Santander, Laura Cubillana-Aguilera

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Mikrochimica acta, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Álvaro Jesús Sainz-Calvo *Institute of Research on Electron Microscopy and Materials (IMEYMAT), Department of Analytical Chemistry, Faculty of Sciences, Campus de Excelencia Internacional del Mar (CEIMAR), University of Cadiz, Campus Universitario de Puerto Real, Polígono del Río San Pedro S/N, 11510, Puerto Real, Cádiz, Spain.
Alfonso Sierra-Padilla *Institute of Research on Electron Microscopy and Materials (IMEYMAT), Department of Analytical Chemistry, Faculty of Sciences, Campus de Excelencia Internacional del Mar (CEIMAR), University of Cadiz, Campus Universitario de Puerto Real, Polígono del Río San Pedro S/N, 11510, Puerto Real, Cádiz, Spain.
Dolores Bellido-MillaInstitute of Research on Electron Microscopy and Materials (IMEYMAT), Department of Analytical Chemistry, Faculty of Sciences, Campus de Excelencia Internacional del Mar (CEIMAR), University of Cadiz, Campus Universitario de Puerto Real, Polígono del Río San Pedro S/N, 11510, Puerto Real, Cádiz, Spain.
Lorena Blanco-DíazInstitute of Research on Electron Microscopy and Materials (IMEYMAT), Department of Analytical Chemistry, Faculty of Sciences, Campus de Excelencia Internacional del Mar (CEIMAR), University of Cadiz, Campus Universitario de Puerto Real, Polígono del Río San Pedro S/N, 11510, Puerto Real, Cádiz, Spain.
Juan Jesús Fernández-AlbaDepartamento de Obstetricia y Ginecología, Hospital Universitario de Puerto Real, Puerto Real, Cádiz, 11510, Spain.
Carmen González-MacíasDepartamento de Obstetricia y Ginecología, Hospital Universitario de Puerto Real, Puerto Real, Cádiz, 11510, Spain.
Juan José García-GuzmánInstitute of Research on Electron Microscopy and Materials (IMEYMAT), Department of Analytical Chemistry, Faculty of Sciences, Campus de Excelencia Internacional del Mar (CEIMAR), University of Cadiz, Campus Universitario de Puerto Real, Polígono del Río San Pedro S/N, 11510, Puerto Real, Cádiz, Spain. juanjo.garciaguzman@uca.es.
José María Palacios-SantanderInstitute of Research on Electron Microscopy and Materials (IMEYMAT), Department of Analytical Chemistry, Faculty of Sciences, Campus de Excelencia Internacional del Mar (CEIMAR), University of Cadiz, Campus Universitario de Puerto Real, Polígono del Río San Pedro S/N, 11510, Puerto Real, Cádiz, Spain. josem.palacios@uca.es.
Laura Cubillana-AguileraInstitute of Research on Electron Microscopy and Materials (IMEYMAT), Department of Analytical Chemistry, Faculty of Sciences, Campus de Excelencia Internacional del Mar (CEIMAR), University of Cadiz, Campus Universitario de Puerto Real, Polígono del Río San Pedro S/N, 11510, Puerto Real, Cádiz, Spain.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Enzyme saturation in biosensing has been established as a main hindrance in lactate monitoring due to the high concentration of this species in biological samples. In this paper, a Sonogel-carbon transducer has been used for the fabrication of an amperometric biosensor for lactate (LA) determination. The sensor was constructed by immobilizing lactate oxidase as the bioreceptor and coating the electrode surface with multipolymer layers to minimize enzyme saturation and improve operational stability. This biosensor shows adequate sensitivity (1.74 µA mM

Indexed as

Biosensing TechniquesElectrochemical TechniquesLactic AcidPrinting, Three-DimensionalElectrodesEnzymes, ImmobilizedHumansLimit of DetectionMixed Function OxygenasesPolyethylene GlycolsEnzymes, Immobilizedlactate 2-monooxygenaseLactic AcidMixed Function OxygenasesPolyethylene Glycols3D printed microfluidic cellAmperometryEnzyme encapsulationReal-time lactate monitoringSonogel–carbon-based lactate biosensorUntreated serum analysis

Identifiers

PMID41454948
PMCPMC12743661

What Socratic holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the Socratic graph.