ArticleJournal of orthopaedic surgery and research2025
The clinical significance of miR-18a-3p in traumatic spinal cord injury and its functional role in mediating apoptosis, inflammation and oxidative stress.
Article in Journal of orthopaedic surgery and research, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
backgroundTraumatic spinal cord injury (TSCI) is a traumatic disease of the central nervous system that places a heavy burden on families and society. MicroRNAs (miRNAs) are intimately related to the pathophysiological processes of TSCI. This study aimed to investigate the diagnostic performance of miR-18a-3p and its molecular mechanism in TSCI.
methodsThe 218 patients with acute spinal trauma were classified according to the ASIA criteria into a normal group (n = 68), an incomplete SCI (ISCI) group (n = 85), and a complete SCI (CSCI) group (n = 65). Serum samples from participants were used to evaluate the role of miR-18a-3p in clinical diagnosis. The levels of miR-18a-3p were detected by RT-qPCR. ROC curve analysis evaluated the diagnostic performance of miR-18a-3p for TSCI. PC12 cells, which was a cell line widely used in vitro neuronal models, were treated with 5 µg/mL LPS for 12 h to simulate an inflammatory environment. Cell viability and apoptosis were assessed via CCK-8 assays and flow cytometry. Inflammatory cytokine levels were measured by ELISA. miR-18a-3p target sites were predicted using the TargetScan database. Target relationships were validated through dual-luciferase reporter assays.
resultsCompared with the Normal group, the levels of miR-18a-3p were approximately 0.4-fold higher in ISCI patients and approximately 0.7-fold higher in CSCI patients. ROC curve analysis revealed that miR-18a-3p had good diagnostic value for TSCI. It also distinguished between ISCI and CSCI patients. Additionally, the content of miR-18a-3p in patient serum had positive correlations with inflammatory cytokines (TNF-α, IL-1β, and IL-6). DLST was a target site of miR-18a-3p. In cell models, transfection with miR-18a-3p inhibitor enhanced cell viability and the levels of SOD and GSH. Meanwhile it suppressed proliferation, inflammatory factors and MDA levels. si-DLST attenuated the effects of the miR-18a-3p inhibitor.
conclusionmiR-18a-3p may be a potential diagnostic marker for TSCI. Mechanistically, downregulated miR-18a-3p inhibits apoptosis, inflammation and oxidative stress by negatively modulating DLST, thereby mitigating the progression of TSCI.
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